Abstract
Cotinine is a major metabolite of nicotine and serves as an important biomarker of tobacco smoke exposure. To monitor exposure to tobacco smoke or nicotine, a sensitive enzyme-linked immunosorbent assay (ELISA) for cotinine was developed. The test had an 1-50 of between 0.5 and 1.0 ng/ml for cotinine and about 500-fold less affinity for nicotine. Few matrix effects were not detectable in human saliva, although relatively small matrix effects (1-50 for cotinine, 2.8 ng/ml) were observed in human serum and urine. The test accurately measured the levels of cotinine in NI5T standards in freeze-dried human urine derivative material (r =9999) indicating its reliability for measurement of cotinine. The test readily detected low levels (5-500 nglml) of cotinine in human saliva and serum samples. Also, the levels of cotinine in plasma and urine samples from smoke-exposed mice and rats could be rapidly analyzed for cotinine. This ELISA is therefore a sensitive and accurate test for the determination of cotinine in plasma, serum, saliva, and urine samples from humans and animals, and can be successfully used for monitoring and quantifying exposure to tobacco smoke or nicotine.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 403-413 |
| Number of pages | 11 |
| Journal | Inhalation Toxicology |
| Volume | 5 |
| Issue number | 4 |
| DOIs | |
| State | Published - 1993 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Toxicology
- Health, Toxicology and Mutagenesis
Cite this
Development and characterization of an elisa for cotinine in biological fluids. / Stanley, Scott D; Gairola, C. G.; Diana, J.; Huffman, M.; Sadove, R.; Woods, W. E.; Kwiatkowski, S.; Tai, H. H.; Tobin, T.
In: Inhalation Toxicology, Vol. 5, No. 4, 1993, p. 403-413.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Development and characterization of an elisa for cotinine in biological fluids
AU - Stanley, Scott D
AU - Gairola, C. G.
AU - Diana, J.
AU - Huffman, M.
AU - Sadove, R.
AU - Woods, W. E.
AU - Kwiatkowski, S.
AU - Tai, H. H.
AU - Tobin, T.
PY - 1993
Y1 - 1993
N2 - Cotinine is a major metabolite of nicotine and serves as an important biomarker of tobacco smoke exposure. To monitor exposure to tobacco smoke or nicotine, a sensitive enzyme-linked immunosorbent assay (ELISA) for cotinine was developed. The test had an 1-50 of between 0.5 and 1.0 ng/ml for cotinine and about 500-fold less affinity for nicotine. Few matrix effects were not detectable in human saliva, although relatively small matrix effects (1-50 for cotinine, 2.8 ng/ml) were observed in human serum and urine. The test accurately measured the levels of cotinine in NI5T standards in freeze-dried human urine derivative material (r =9999) indicating its reliability for measurement of cotinine. The test readily detected low levels (5-500 nglml) of cotinine in human saliva and serum samples. Also, the levels of cotinine in plasma and urine samples from smoke-exposed mice and rats could be rapidly analyzed for cotinine. This ELISA is therefore a sensitive and accurate test for the determination of cotinine in plasma, serum, saliva, and urine samples from humans and animals, and can be successfully used for monitoring and quantifying exposure to tobacco smoke or nicotine.
AB - Cotinine is a major metabolite of nicotine and serves as an important biomarker of tobacco smoke exposure. To monitor exposure to tobacco smoke or nicotine, a sensitive enzyme-linked immunosorbent assay (ELISA) for cotinine was developed. The test had an 1-50 of between 0.5 and 1.0 ng/ml for cotinine and about 500-fold less affinity for nicotine. Few matrix effects were not detectable in human saliva, although relatively small matrix effects (1-50 for cotinine, 2.8 ng/ml) were observed in human serum and urine. The test accurately measured the levels of cotinine in NI5T standards in freeze-dried human urine derivative material (r =9999) indicating its reliability for measurement of cotinine. The test readily detected low levels (5-500 nglml) of cotinine in human saliva and serum samples. Also, the levels of cotinine in plasma and urine samples from smoke-exposed mice and rats could be rapidly analyzed for cotinine. This ELISA is therefore a sensitive and accurate test for the determination of cotinine in plasma, serum, saliva, and urine samples from humans and animals, and can be successfully used for monitoring and quantifying exposure to tobacco smoke or nicotine.
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U2 - 10.3109/08958379308998395
DO - 10.3109/08958379308998395
M3 - Article
VL - 5
SP - 403
EP - 413
JO - Inhalation Toxicology
JF - Inhalation Toxicology
SN - 0895-8378
IS - 4
ER -