Development and characterization of an elisa for cotinine in biological fluids

Scott D Stanley, C. G. Gairola, J. Diana, M. Huffman, R. Sadove, W. E. Woods, S. Kwiatkowski, H. H. Tai, T. Tobin

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Cotinine is a major metabolite of nicotine and serves as an important biomarker of tobacco smoke exposure. To monitor exposure to tobacco smoke or nicotine, a sensitive enzyme-linked immunosorbent assay (ELISA) for cotinine was developed. The test had an 1-50 of between 0.5 and 1.0 ng/ml for cotinine and about 500-fold less affinity for nicotine. Few matrix effects were not detectable in human saliva, although relatively small matrix effects (1-50 for cotinine, 2.8 ng/ml) were observed in human serum and urine. The test accurately measured the levels of cotinine in NI5T standards in freeze-dried human urine derivative material (r =9999) indicating its reliability for measurement of cotinine. The test readily detected low levels (5-500 nglml) of cotinine in human saliva and serum samples. Also, the levels of cotinine in plasma and urine samples from smoke-exposed mice and rats could be rapidly analyzed for cotinine. This ELISA is therefore a sensitive and accurate test for the determination of cotinine in plasma, serum, saliva, and urine samples from humans and animals, and can be successfully used for monitoring and quantifying exposure to tobacco smoke or nicotine.

Original languageEnglish (US)
Pages (from-to)403-413
Number of pages11
JournalInhalation Toxicology
Volume5
Issue number4
DOIs
StatePublished - 1993
Externally publishedYes

Fingerprint

Cotinine
Fluids
Nicotine
Smoke
Tobacco
Saliva
Urine
Immunosorbents
Assays
Serum
Enzyme-Linked Immunosorbent Assay
Plasmas
Biomarkers
Enzymes
Metabolites
Rats
Animals
Derivatives

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Stanley, S. D., Gairola, C. G., Diana, J., Huffman, M., Sadove, R., Woods, W. E., ... Tobin, T. (1993). Development and characterization of an elisa for cotinine in biological fluids. Inhalation Toxicology, 5(4), 403-413. https://doi.org/10.3109/08958379308998395

Development and characterization of an elisa for cotinine in biological fluids. / Stanley, Scott D; Gairola, C. G.; Diana, J.; Huffman, M.; Sadove, R.; Woods, W. E.; Kwiatkowski, S.; Tai, H. H.; Tobin, T.

In: Inhalation Toxicology, Vol. 5, No. 4, 1993, p. 403-413.

Research output: Contribution to journalArticle

Stanley, SD, Gairola, CG, Diana, J, Huffman, M, Sadove, R, Woods, WE, Kwiatkowski, S, Tai, HH & Tobin, T 1993, 'Development and characterization of an elisa for cotinine in biological fluids', Inhalation Toxicology, vol. 5, no. 4, pp. 403-413. https://doi.org/10.3109/08958379308998395
Stanley, Scott D ; Gairola, C. G. ; Diana, J. ; Huffman, M. ; Sadove, R. ; Woods, W. E. ; Kwiatkowski, S. ; Tai, H. H. ; Tobin, T. / Development and characterization of an elisa for cotinine in biological fluids. In: Inhalation Toxicology. 1993 ; Vol. 5, No. 4. pp. 403-413.
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