Development and characterization of 15 polymorphic microsatellite markers for North Pacific albatrosses using paired-end Illumina shotgun sequencing

Luis D. Hernandez, Zachary Lounsberry, Paul W. Collins, Robert W. Henry, Seth D. Newsome, Benjamin Sacks

Research output: Contribution to journalLetter

Abstract

All three species of North Pacific albatross are vulnerable or near threatened (IUCN 2013) yet microsatellite markers to study their population characteristics are lacking. We employed 250-bp paired-end whole genome shotgun sequencing on an Illumina MiSeq platform to discover microsatellite markers. We selected 16 candidate loci for screening in 36 Black-footed (Phoebastria nigripes), 12 Laysan (P. immutabilis), and 3 Short-tailed (P. albatrus) albatrosses from which we characterized 15 markers.

Original languageEnglish (US)
Pages (from-to)491-493
Number of pages3
JournalConservation Genetics Resources
Volume6
Issue number2
DOIs
StatePublished - Jan 1 2014

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Keywords

  • Albatross
  • Illumina
  • Microsatellite
  • PAL FINDER
  • PCR primers
  • Phoebastria

ASJC Scopus subject areas

  • Genetics
  • Ecology, Evolution, Behavior and Systematics

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