Development and characterization of 15 polymorphic microsatellite markers for North Pacific albatrosses using paired-end Illumina shotgun sequencing

Luis D. Hernandez, Zachary Lounsberry, Paul W. Collins, Robert W. Henry, Seth D. Newsome, Benjamin Sacks

Research output: Contribution to journalLetter

Abstract

All three species of North Pacific albatross are vulnerable or near threatened (IUCN 2013) yet microsatellite markers to study their population characteristics are lacking. We employed 250-bp paired-end whole genome shotgun sequencing on an Illumina MiSeq platform to discover microsatellite markers. We selected 16 candidate loci for screening in 36 Black-footed (Phoebastria nigripes), 12 Laysan (P. immutabilis), and 3 Short-tailed (P. albatrus) albatrosses from which we characterized 15 markers.

Original languageEnglish (US)
Pages (from-to)491-493
Number of pages3
JournalConservation Genetics Resources
Volume6
Issue number2
DOIs
StatePublished - Jan 1 2014

Fingerprint

Firearms
Microsatellite Repeats
sequence analysis
microsatellite repeats
population characteristics
Population Characteristics
Genome
screening
loci
genome
marker
Phoebastria
Phoebastria albatrus

Keywords

  • Albatross
  • Illumina
  • Microsatellite
  • PAL FINDER
  • PCR primers
  • Phoebastria

ASJC Scopus subject areas

  • Genetics
  • Ecology, Evolution, Behavior and Systematics

Cite this

Development and characterization of 15 polymorphic microsatellite markers for North Pacific albatrosses using paired-end Illumina shotgun sequencing. / Hernandez, Luis D.; Lounsberry, Zachary; Collins, Paul W.; Henry, Robert W.; Newsome, Seth D.; Sacks, Benjamin.

In: Conservation Genetics Resources, Vol. 6, No. 2, 01.01.2014, p. 491-493.

Research output: Contribution to journalLetter

@article{8183ac6f5c5e422b8df0fe4e524b9c22,
title = "Development and characterization of 15 polymorphic microsatellite markers for North Pacific albatrosses using paired-end Illumina shotgun sequencing",
abstract = "All three species of North Pacific albatross are vulnerable or near threatened (IUCN 2013) yet microsatellite markers to study their population characteristics are lacking. We employed 250-bp paired-end whole genome shotgun sequencing on an Illumina MiSeq platform to discover microsatellite markers. We selected 16 candidate loci for screening in 36 Black-footed (Phoebastria nigripes), 12 Laysan (P. immutabilis), and 3 Short-tailed (P. albatrus) albatrosses from which we characterized 15 markers.",
keywords = "Albatross, Illumina, Microsatellite, PAL FINDER, PCR primers, Phoebastria",
author = "Hernandez, {Luis D.} and Zachary Lounsberry and Collins, {Paul W.} and Henry, {Robert W.} and Newsome, {Seth D.} and Benjamin Sacks",
year = "2014",
month = "1",
day = "1",
doi = "10.1007/s12686-013-0138-8",
language = "English (US)",
volume = "6",
pages = "491--493",
journal = "Conservation Genetics Resources",
issn = "1877-7252",
publisher = "Springer Verlag",
number = "2",

}

TY - JOUR

T1 - Development and characterization of 15 polymorphic microsatellite markers for North Pacific albatrosses using paired-end Illumina shotgun sequencing

AU - Hernandez, Luis D.

AU - Lounsberry, Zachary

AU - Collins, Paul W.

AU - Henry, Robert W.

AU - Newsome, Seth D.

AU - Sacks, Benjamin

PY - 2014/1/1

Y1 - 2014/1/1

N2 - All three species of North Pacific albatross are vulnerable or near threatened (IUCN 2013) yet microsatellite markers to study their population characteristics are lacking. We employed 250-bp paired-end whole genome shotgun sequencing on an Illumina MiSeq platform to discover microsatellite markers. We selected 16 candidate loci for screening in 36 Black-footed (Phoebastria nigripes), 12 Laysan (P. immutabilis), and 3 Short-tailed (P. albatrus) albatrosses from which we characterized 15 markers.

AB - All three species of North Pacific albatross are vulnerable or near threatened (IUCN 2013) yet microsatellite markers to study their population characteristics are lacking. We employed 250-bp paired-end whole genome shotgun sequencing on an Illumina MiSeq platform to discover microsatellite markers. We selected 16 candidate loci for screening in 36 Black-footed (Phoebastria nigripes), 12 Laysan (P. immutabilis), and 3 Short-tailed (P. albatrus) albatrosses from which we characterized 15 markers.

KW - Albatross

KW - Illumina

KW - Microsatellite

KW - PAL FINDER

KW - PCR primers

KW - Phoebastria

UR - http://www.scopus.com/inward/record.url?scp=84900804349&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84900804349&partnerID=8YFLogxK

U2 - 10.1007/s12686-013-0138-8

DO - 10.1007/s12686-013-0138-8

M3 - Letter

VL - 6

SP - 491

EP - 493

JO - Conservation Genetics Resources

JF - Conservation Genetics Resources

SN - 1877-7252

IS - 2

ER -