Development and characterization of 15 polymorphic microsatellite markers for North Pacific albatrosses using paired-end Illumina shotgun sequencing

Luis D. Hernandez, Zachary Lounsberry, Paul W. Collins, Robert W. Henry, Seth D. Newsome, Benjamin Sacks

Research output: Contribution to journalLetterpeer-review

Abstract

All three species of North Pacific albatross are vulnerable or near threatened (IUCN 2013) yet microsatellite markers to study their population characteristics are lacking. We employed 250-bp paired-end whole genome shotgun sequencing on an Illumina MiSeq platform to discover microsatellite markers. We selected 16 candidate loci for screening in 36 Black-footed (Phoebastria nigripes), 12 Laysan (P. immutabilis), and 3 Short-tailed (P. albatrus) albatrosses from which we characterized 15 markers.

Original languageEnglish (US)
Pages (from-to)491-493
Number of pages3
JournalConservation Genetics Resources
Volume6
Issue number2
DOIs
StatePublished - Jan 1 2014

Keywords

  • Albatross
  • Illumina
  • Microsatellite
  • PAL FINDER
  • PCR primers
  • Phoebastria

ASJC Scopus subject areas

  • Genetics
  • Ecology, Evolution, Behavior and Systematics

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