Development and characterization of 15 polymorphic dinucleotide microsatellite markers for tule elk using hiseq3000

Benjamin Sacks, Zachary Lounsberry, Tatyana Kalani, Erin P. Meredith, Cristen Langner

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The tule elk (Cervus elaphus nannodes) experienced a severe bottleneck in the 1800s, resulting in low genetic diversity. There is a need for high-resolution genetic assays that can be used to differentiate individual elk, including close relatives, with high confidence. An efficient assay requires multiple markers both polymorphic and that can be amplified in concert with other markers in multiplex reactions. To develop such markers, we employed 150-bp paired-end whole genome shotgun sequencing on an Illumina HiSeq3000 platform to discover dinucleotide microsatellite markers. After preliminary screening of these markers, we selected and screened 15 candidate loci and 5 existing tetra nucleotide markers in 56 tule elk. We combined these markers in 2 multiplex reactions and report primer concentrations and PCR conditions enabling their efficient amplification.

Original languageEnglish (US)
Pages (from-to)666-669
Number of pages4
JournalJournal of Heredity
Volume107
Issue number7
DOIs
StatePublished - Jan 1 2016

Keywords

  • Cervus elaphus nannodes
  • dinucleotide
  • HiSeq
  • Illumina
  • microsatellite
  • tule elk

ASJC Scopus subject areas

  • Biotechnology
  • Molecular Biology
  • Genetics
  • Genetics(clinical)

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