Development and application of patch-clamp fluorometry in life science research

Wei Cheng, Mei Li, Sha Du, Jie Zheng

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Patch-clamp fluorometry is a powerful biophysical method that allows the researcher to monitor conformational changes of ion channel in real time, and to correlate dynamic structural information to functional states. As a natural combination of the classic patch-clamping method and state-of-art optical methods, patch-clamp fluorometry is applicable to a wide spectrum of channel studies with unprecedent spatial-temporal resolutions. Unlike other structure-oriented methods such as X-ray crystallography and cryo-EM, patch-clamp fluorometry provides dynamic structural information of channel proteins in intact cell membrane under physiological conditions.

Original languageEnglish (US)
Pages (from-to)187-192
Number of pages6
JournalProgress in Biochemistry and Biophysics
Volume43
Issue number2
DOIs
StatePublished - Feb 1 2016
Externally publishedYes

Keywords

  • Conformational change
  • Fluorophore
  • Gating
  • Membrane protein
  • Patch-clamp fluorometry

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry

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