Developing rapid, point-of-care, multiplex detection for use in lateral flow devices

R. S. Rao; J. S. Albala; S. L. Lane; Dennis L Matthews; A. M. Fisher; J. L. Lambert; Matthew A Coleman

doi:10.1117/12.630157

Developing rapid, point-of-care, multiplex detection for use in lateral flow devices

R. S. Rao, J. S. Albala, S. L. Lane, Dennis L Matthews, A. M. Fisher, J. L. Lambert, Matthew A Coleman

Neurological Surgery

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

4 Scopus citations

Abstract

Immunoassays have been widely used in commercial, scientific and medical research for detection and quantification of analytes in complex mixtures. There is however a need for a point-of-care, multiplex diagnostic assays capable of providing rapid and quantitative measurements of analytes present in samples that are sufficiently simple to carry out without use of a laboratory or individuals trained in chemical analysis. We are developing a fluorescent lateral flow immunoassay platform to perform simultaneous, multiplexed detection of analytes in a complex fluid mixture along with instrumentation to optically quantitate the analytes in the sample. Our prototype imaging system is based on conventional 16-bit CCD optics, which enables the development of a rugged diagnostic instrument that can be further scaled down for point-of-care applications. We have compared protein microarrays with lateral flow assays (LFAs) to determine the sensitivity of each system for the measurement of distinct proteins in complex samples. We are pursuing the LFA platform such that it can easily be scaled to meet the requirements of any given screening application, and be implemented for use in a medical or surgical setting.

Original language	English (US)
Title of host publication	Proceedings of SPIE - The International Society for Optical Engineering
Editors	B.M. Cullum, J.C. Carter
Volume	6007
DOIs	https://doi.org/10.1117/12.630157
State	Published - 2005
Event	Smart Medical and Biomedical Sensor Technology III - Boston, MA, United States Duration: Oct 24 2005 → Oct 26 2005

Other

Other	Smart Medical and Biomedical Sensor Technology III
Country	United States
City	Boston, MA
Period	10/24/05 → 10/26/05

Keywords

Cancer diagnostics
Immunoassay
Lateral flow assay
Microarray

ASJC Scopus subject areas

Electrical and Electronic Engineering
Condensed Matter Physics

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10.1117/12.630157

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Developing rapid, point-of-care, multiplex detection for use in lateral flow devices. / Rao, R. S.; Albala, J. S.; Lane, S. L.; Matthews, Dennis L; Fisher, A. M.; Lambert, J. L.; Coleman, Matthew A.

Proceedings of SPIE - The International Society for Optical Engineering. ed. / B.M. Cullum; J.C. Carter. Vol. 6007 2005. 600711.

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Rao, RS, Albala, JS, Lane, SL, Matthews, DL, Fisher, AM, Lambert, JL & Coleman, MA 2005, Developing rapid, point-of-care, multiplex detection for use in lateral flow devices. in BM Cullum & JC Carter (eds), Proceedings of SPIE - The International Society for Optical Engineering. vol. 6007, 600711, Smart Medical and Biomedical Sensor Technology III, Boston, MA, United States, 10/24/05. https://doi.org/10.1117/12.630157

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abstract = "Immunoassays have been widely used in commercial, scientific and medical research for detection and quantification of analytes in complex mixtures. There is however a need for a point-of-care, multiplex diagnostic assays capable of providing rapid and quantitative measurements of analytes present in samples that are sufficiently simple to carry out without use of a laboratory or individuals trained in chemical analysis. We are developing a fluorescent lateral flow immunoassay platform to perform simultaneous, multiplexed detection of analytes in a complex fluid mixture along with instrumentation to optically quantitate the analytes in the sample. Our prototype imaging system is based on conventional 16-bit CCD optics, which enables the development of a rugged diagnostic instrument that can be further scaled down for point-of-care applications. We have compared protein microarrays with lateral flow assays (LFAs) to determine the sensitivity of each system for the measurement of distinct proteins in complex samples. We are pursuing the LFA platform such that it can easily be scaled to meet the requirements of any given screening application, and be implemented for use in a medical or surgical setting.",

keywords = "Cancer diagnostics, Immunoassay, Lateral flow assay, Microarray",

author = "Rao, {R. S.} and Albala, {J. S.} and Lane, {S. L.} and Matthews, {Dennis L} and Fisher, {A. M.} and Lambert, {J. L.} and Coleman, {Matthew A}",

year = "2005",

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AU - Fisher, A. M.

AU - Lambert, J. L.

AU - Coleman, Matthew A

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AB - Immunoassays have been widely used in commercial, scientific and medical research for detection and quantification of analytes in complex mixtures. There is however a need for a point-of-care, multiplex diagnostic assays capable of providing rapid and quantitative measurements of analytes present in samples that are sufficiently simple to carry out without use of a laboratory or individuals trained in chemical analysis. We are developing a fluorescent lateral flow immunoassay platform to perform simultaneous, multiplexed detection of analytes in a complex fluid mixture along with instrumentation to optically quantitate the analytes in the sample. Our prototype imaging system is based on conventional 16-bit CCD optics, which enables the development of a rugged diagnostic instrument that can be further scaled down for point-of-care applications. We have compared protein microarrays with lateral flow assays (LFAs) to determine the sensitivity of each system for the measurement of distinct proteins in complex samples. We are pursuing the LFA platform such that it can easily be scaled to meet the requirements of any given screening application, and be implemented for use in a medical or surgical setting.

KW - Cancer diagnostics

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KW - Lateral flow assay

KW - Microarray

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Developing rapid, point-of-care, multiplex detection for use in lateral flow devices

Abstract

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Keywords

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