Developing an efficient and general strategy for immobilization of small molecules onto microarrays using isocyanate chemistry

Chenggang Zhu, Xiangdong Zhu, James P. Landry, Zhaomeng Cui, Quanfu Li, Yongjun Dang, Lan Mi, Fengyun Zheng, Yiyan Fei

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

Small-molecule microarray (SMM) is an effective platform for identifying lead compounds from large collections of small molecules in drug discovery, and efficient immobilization of molecular compounds is a pre-requisite for the success of such a platform. On an isocyanate functionalized surface, we studied the dependence of immobilization efficiency on chemical residues on molecular compounds, terminal residues on isocyanate functionalized surface, lengths of spacer molecules, and post-printing treatment conditions, and we identified a set of optimized conditions that enable us to immobilize small molecules with significantly improved efficiencies, particularly for those molecules with carboxylic acid residues that are known to have low isocyanate reactivity. We fabricated microarrays of 3375 bioactive compounds on isocyanate functionalized glass slides under these optimized conditions and confirmed that immobilization percentage is over 73%.

Original languageEnglish (US)
Article number378
JournalSensors (Switzerland)
Volume16
Issue number3
DOIs
StatePublished - Mar 16 2016

Keywords

  • High-throughput screening
  • Label-free optical biosensor
  • Small-molecule microarrays
  • Surface functionalization

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Atomic and Molecular Physics, and Optics
  • Analytical Chemistry
  • Biochemistry

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