Developing an articular cartilage decellularization process toward facet joint cartilage replacement

Benjamin D. Elder, Daniel H. Kim, Kyriacos A. Athanasiou

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

OBJECTIVE: The facet joint has been identified as a significant source of morbidity in lower back pain. In general, treatments have focused on reducing the pain associated with facet joint osteoarthritis, and no treatments have targeted the development of a replacement tissue for arthritic facet articular cartilage. Therefore, the objective of this study was to develop a nonimmunogenic decellularized articular cartilage replacement tissue while maintaining functional properties similar to native facet cartilage tissue. METHODS: In vitro testing was performed on bovine articular cartilage explants. The effects of 2% sodium dodecyl sulfate (SDS), a detergent used for cell and nuclear membrane solubilization, on cartilage cellularity, biochemical, and biomechanical properties, were examined. Compressive biomechanical properties were determined using creep indentation, and the tensile biomechanical properties were obtained with uniaxial tensile testing. Biochemical assessment involved determination of the DNA content, glycosaminoglycan (GAG) content, and collagen content. Histological examination included hematoxylin and eosin staining for tissue cellularity, as well as staining for collagen and GAG. RESULTS: Treatment with 2% SDS for 2 hours maintained the compressive and tensile biomechanical properties, as well as the GAG and collagen content while resulting in a decrease in cell nuclei and a 4% decrease in DNA content. Additionally, treatment for 8 hours resulted in complete histological decellularization and a 40% decrease in DNA content while maintaining collagen content and tensile properties. However, a significant decrease in compressive properties and GAG content was observed. Similar results were observed with 4 hours of treatment, although the decrease in DNA content was not as great as with 8 hours of treatment. CONCLUSION: Treatment with 2% SDS for 8 hours resulted in complete histological decellularization with decreased mechanical properties, whereas treatment for 2 hours maintained mechanical properties, but had a minimal effect on DNA content. Therefore, future studies must be performed to optimize a treatment for decellularization while maintaining mechanical properties close to those of facet joint cartilage. This study served as a step in creating a decellularized articular cartilage replacement tissue that could be used as a treatment for facet cartilage osteoarthritis.

Original languageEnglish (US)
Pages (from-to)722-727
Number of pages6
JournalNeurosurgery
Volume66
Issue number4
DOIs
StatePublished - Apr 2010

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Replacement Arthroplasties
Zygapophyseal Joint
Articular Cartilage
Cartilage
Glycosaminoglycans
Collagen
Sodium Dodecyl Sulfate
DNA
Osteoarthritis
Staining and Labeling
Therapeutics
Nuclear Envelope
Hematoxylin
Eosine Yellowish-(YS)
Low Back Pain
Cell Nucleus
Detergents
Arthritis
Cell Membrane
Morbidity

Keywords

  • Biomechanics
  • Cartilage
  • Decellularization
  • Extracellular matrix
  • Facet joint

ASJC Scopus subject areas

  • Clinical Neurology
  • Surgery

Cite this

Developing an articular cartilage decellularization process toward facet joint cartilage replacement. / Elder, Benjamin D.; Kim, Daniel H.; Athanasiou, Kyriacos A.

In: Neurosurgery, Vol. 66, No. 4, 04.2010, p. 722-727.

Research output: Contribution to journalArticle

Elder, Benjamin D. ; Kim, Daniel H. ; Athanasiou, Kyriacos A. / Developing an articular cartilage decellularization process toward facet joint cartilage replacement. In: Neurosurgery. 2010 ; Vol. 66, No. 4. pp. 722-727.
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abstract = "OBJECTIVE: The facet joint has been identified as a significant source of morbidity in lower back pain. In general, treatments have focused on reducing the pain associated with facet joint osteoarthritis, and no treatments have targeted the development of a replacement tissue for arthritic facet articular cartilage. Therefore, the objective of this study was to develop a nonimmunogenic decellularized articular cartilage replacement tissue while maintaining functional properties similar to native facet cartilage tissue. METHODS: In vitro testing was performed on bovine articular cartilage explants. The effects of 2{\%} sodium dodecyl sulfate (SDS), a detergent used for cell and nuclear membrane solubilization, on cartilage cellularity, biochemical, and biomechanical properties, were examined. Compressive biomechanical properties were determined using creep indentation, and the tensile biomechanical properties were obtained with uniaxial tensile testing. Biochemical assessment involved determination of the DNA content, glycosaminoglycan (GAG) content, and collagen content. Histological examination included hematoxylin and eosin staining for tissue cellularity, as well as staining for collagen and GAG. RESULTS: Treatment with 2{\%} SDS for 2 hours maintained the compressive and tensile biomechanical properties, as well as the GAG and collagen content while resulting in a decrease in cell nuclei and a 4{\%} decrease in DNA content. Additionally, treatment for 8 hours resulted in complete histological decellularization and a 40{\%} decrease in DNA content while maintaining collagen content and tensile properties. However, a significant decrease in compressive properties and GAG content was observed. Similar results were observed with 4 hours of treatment, although the decrease in DNA content was not as great as with 8 hours of treatment. CONCLUSION: Treatment with 2{\%} SDS for 8 hours resulted in complete histological decellularization with decreased mechanical properties, whereas treatment for 2 hours maintained mechanical properties, but had a minimal effect on DNA content. Therefore, future studies must be performed to optimize a treatment for decellularization while maintaining mechanical properties close to those of facet joint cartilage. This study served as a step in creating a decellularized articular cartilage replacement tissue that could be used as a treatment for facet cartilage osteoarthritis.",
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