Determination of the median toxic dose of type C botulinum toxin in lactating dairy cows

Robert B. Moeller, Birgit Puschner, Richard L. Walker, Tonie Rocke, Frank D. Galey, James S Cullor, Alex Ardans

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Because of the difficulty in identifying botulinum toxin in cattle, it is hypothesized that cattle are sensitive to levels of toxin below the detection limits of current diagnostic techniques (the mouse protection bioassay and the immunostick enzyme-linked immunosorbent assay [ELISA] for type C botulinum toxin). Using an up-down method for toxicologic testing, the median toxic dose (MTD50) for cattle was determined. Four lactating Holstein cows were dosed at 0.125 or 0.25 ng/kg with Clostridium botulinum type C toxin and failed to develop clinical signs of botulism during the 7-day observation period. Three cows given 0.50 ng/kg of toxin developed clinical signs of botulism. From these results, the MTD50 was calculated at 0.388 ng/kg (3.88 mouse lethal doses/kg) using the trim-logit method. These results suggest that cattle are 12.88 times more sensitive to type C botulinum toxin than a mouse on a per kilogram weight basis. The mouse protection bioassay and the immunostick ELISA for type C botulinum toxin failed to identify the presence of the toxin in the serum, blood, and milk samples taken from all 7 animals.

Original languageEnglish (US)
Pages (from-to)523-526
Number of pages4
JournalJournal of Veterinary Diagnostic Investigation
Volume15
Issue number6
StatePublished - Nov 2003

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botulinum toxin
Poisons
toxins
dairy cows
Botulism
botulism
cattle
mice
dosage
Biological Assay
bioassays
Enzyme-Linked Immunosorbent Assay
Clostridium botulinum C
enzyme-linked immunosorbent assay
cows
Botulinum Toxins
lethal dose
blood serum
whey
diagnostic techniques

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Determination of the median toxic dose of type C botulinum toxin in lactating dairy cows. / Moeller, Robert B.; Puschner, Birgit; Walker, Richard L.; Rocke, Tonie; Galey, Frank D.; Cullor, James S; Ardans, Alex.

In: Journal of Veterinary Diagnostic Investigation, Vol. 15, No. 6, 11.2003, p. 523-526.

Research output: Contribution to journalArticle

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