Determination of Strophanthidin in Ingesta and Plant Material by LC-MS/MS

Michael S. Filigenzi, Leslie Woods, Marcia C. Booth, Elizabeth R. Tor, Birgit Puschner

Research output: Contribution to journalArticlepeer-review

5 Scopus citations


An LC-MS/MS method was developed for the semiquantitative determination of strophanthidin glycosides in ingesta from animals. Strophanthidin glycosides were simultaneously extracted and hydrolyzed to the strophanthidin aglycone using aqueous methanolic hydrochloric acid and the extracts cleaned up using solid-phase extraction. Extracts were analyzed using reverse-phase HPLC coupled with positive ion electrospray mass spectrometry. Characteristic product ion spectra were produced by fragmentation of the [M + H]+ precursor ion for each analyte. Quantitation was performed using the internal standard method with digitoxigenin serving as the internal standard. The method detection limit was calculated to be 0.075 μg/g, and the limit of quantitation was calculated to be 0.24 μg/g, for strophanthidin in control rumen samples. This method was used in diagnostic investigations to confirm fatal strophanthidin glycoside poisonings in horses.

Original languageEnglish (US)
Pages (from-to)2174-2178
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Issue number8
StatePublished - Apr 21 2004


  • Cardiotoxic glycosides
  • HPLC
  • LC-MS
  • Mass spectrometry
  • Strophanthidin

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)


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