Determination of skin concentrations of enrofloxacin in dogs with pyoderma

T. C. DeManuelle, P. J. Ihrke, C. M. Brandt, Philip H Kass, Philip R Vulliet

Research output: Contribution to journalArticle

24 Scopus citations

Abstract

Objective - To compare serum and skin concentrations of enrofloxacin in dogs with pyoderma with those of clinically normal dogs and to evaluate concentrations in dogs with superficial versus deep pyoderma. Animals - 16 clinically normal dogs and 16 dogs with pyoderma. Procedure - Enrofloxacin (approx 5 mg/kg of body weight, PO) was administered daily to all dogs. Serum samples and skin biopsy specimens were obtained on day 1 at 3 hours after drug administration and on day 3 immediately before and 3 hours after drug administration. Samples and specimens were assayed by high-performance liquid chromatography. Morphometric analysis was performed on skin biopsy specimens to determine correlation between inflammatory cells and peak tissue enrofloxacin concentration on day 1. Results - Morphometric analysis revealed high correlation between dermal inflammatory cell count and drug concentration in dogs with pyoderma. Conclusions - At mean dosage of 5 mg/kg once daily, enrofloxacin tissue concentrations were significantly greater in dogs with pyoderma at 3 hours after pill administration. Enrofloxacin tissue concentration on day 3 at 3 hours after pill administration was 12.4 times the 90% minimum, inhibitory concentration of enrofloxacin for Staphylococcus intermedius. Clinical Relevance - In dogs with pyoderma, therapeutic tissue concentrations of enrofloxacin are reached as early as 3 hours after drug administration.

Original languageEnglish (US)
Pages (from-to)1599-1604
Number of pages6
JournalAmerican Journal of Veterinary Research
Volume59
Issue number12
StatePublished - Dec 1998

ASJC Scopus subject areas

  • veterinary(all)

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