Determination of pyrethroid residues in agricultural products by an enzyme-linked immunosorbent assay

Eun Kee Park, Jung Hyun Kim, Shirley J. Gee, Takaho Watanabe, Ki Chang Ahn, Bruce D. Hammock

Research output: Contribution to journalArticlepeer-review

35 Scopus citations


To determine cypermethrin and permethrin in agricultural products, a competitive enzyme-linked immunosorbent assay (ELISA) method was employed. The matrix interferences were minimized by direct dilution of the extracts. No further cleanup was needed. A minimum matrix effect with a 1:10 dilution of white wine for cypermethrin and a 1:200 dilution of red and white wines, fruits, and vegetables for permethrin was found when phosphate-buffered saline containing 40% methanol was employed as the diluent. Good recoveries of spiked levels were observed. The mean percentage recoveries of cypermethrin spiked in white wine and permethrin spiked in red and white wines were 99.7, 74, and 78%, respectively. The mean percentage recoveries of permethrin spiked in apple, banana, cucumber, lettuce, onion, and peach were 99.2, 105, 70.2, 97.5, 94.4, and 89.4%, respectively. Validation of the ELISA method with permethrin-spiked lettuce and peach was carried out using gas chromatography with mass spectrometry, resulting in a good recovery and correlation.

Original languageEnglish (US)
Pages (from-to)5572-5576
Number of pages5
JournalJournal of Agricultural and Food Chemistry
Issue number18
StatePublished - Sep 8 2004


  • Cypermethrin
  • Environmental monitoring
  • Fruits
  • Immunoassay
  • Permethrin
  • Pyrethroid
  • Vegetables
  • Wine

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Food Science
  • Chemistry (miscellaneous)


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