Determination of N 2-hydroxymethyl-dG adducts in the nasal epithelium and bone marrow of nonhuman primates following 13CD 2-formaldehyde inhalation exposure

Benjamin Moeller, Kun Lu, Melanie Doyle-Eisele, Jacob McDonald, Andrew Gigliotti, James A. Swenberg

Research output: Contribution to journalArticle

60 Scopus citations

Abstract

The presence of endogenous and exogenous N2-hydroxymethyl-dG adducts in DNA from the nasal mucosa and bone marrow of cynomolgus macaques exposed to 1.9 and 6.1 ppm of [13CD2]-formaldehyde for 6 h a day for 2 consecutive days was investigated using a highly sensitive nano-UPLC-MS/MS method with a limit of detection of 20 amol. Both exogenous and endogenous adducts were readily detected and quantified in the nasal tissues of both exposure groups, with an exposure dependent increase in exogenous adducts observed. In contrast, only endogenous adducts were detectable in the bone marrow, even though ∼10 times more DNA was analyzed.

Original languageEnglish (US)
Pages (from-to)162-164
Number of pages3
JournalChemical Research in Toxicology
Volume24
Issue number2
DOIs
StatePublished - Feb 18 2011
Externally publishedYes

ASJC Scopus subject areas

  • Toxicology

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