Determination of hydroperoxides and structures by high-performance liquid chromatography with post-column detection with diphenyl-1- pyrenylphosphine

Toshiaki Ohshima, Anu Hopia, J. Bruce German, Edwin N. Frankel

Research output: Contribution to journalArticle

50 Scopus citations

Abstract

A high-performance liquid chromatographic method, using post-column detection with diphenyl-1-pyrenylphosphine (DPPP), was developed for the quantitative and qualitative determinatiion of isomeric lipid hydroperoxides (OOH). The OOH eluted from a normal-phase column were passed through a photodiode array detector and then mixed with DPPP solution in a reaction coil heated at 80°C. DPPP oxide formed by the reaction with OOH was determined by monitoring the fluorescence intensity at 380 nm and excitation at 352 nm. The conjugated diend OOH (13-cis,trans- and 9-cis,trans-OOH) and nonconjugated OOH (12-cis-trans- and 10-cis,trans-OOH) from photosensitized oxidation of methyl linoleate were determined in a molar ratio of 31:27;19:21, respectively. However, only the two conjugated hydroperoxides were detected by ultraviolet absorption at 234 nm. Further applications were carried out for the determination of OOH of methyl oleate and methyl linolenate. This method proved to be useful for the determination of the OOH containing both conjugated and nonconjugated diene structures.

Original languageEnglish (US)
Pages (from-to)1091-1096
Number of pages6
JournalLipids
Volume31
Issue number10
StatePublished - Oct 1996

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Food Science
  • Biochemistry

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