Determination of glycosylation sites and site-specific heterogeneity in glycoproteins

Hyun Joo An, John W. Froehlich, Carlito B Lebrilla

Research output: Contribution to journalArticlepeer-review

148 Scopus citations


Glycosylation is one of the most common post-translational modifications (PTMs) of proteins. At least 50% of human proteins are glycosylated with some estimates being as high as 70%. Glycoprotein analysis requires determining both the sites of glycosylation as well as the glycan structures associated with each site. Recent advances have led to the development of new analytical methods that employ mass spectrometry extensively making it possible to obtain the glycosylation site and the site microheterogeneity. These tools will be important for the eventual development of glycoproteomics.

Original languageEnglish (US)
Pages (from-to)421-426
Number of pages6
JournalCurrent Opinion in Chemical Biology
Issue number4
StatePublished - Oct 2009

ASJC Scopus subject areas

  • Biochemistry
  • Analytical Chemistry


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