Determination of genetic status in a closed colony of rhesus monkeys (Macaca mulatta)

Marcia C Ribeiro Andrade, Cecilia Penedo, Thea Ward, Virgilio F. Silva, Luciana R. Bertolini, Jeffrey A Roberts, Jose Paulo G Paulo, Pedro H. Cabello

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

The long-term management of breeding colonies requires some measure of genetic diversity in the animal population. For the maintenance of breeding colonies of monkeys used for biomedical research, known pedigrees supply precise data to determine the genetic status of colonies. We present data of genetic analyses in an old closed colony of rhesus macaques (Macaca mulatta) that was established in 1932 with 100 animals. For more than 40 years, the animals were kept on an isolated island and, in 1980, single-male breeding groups were established. A total of 333 DNA samples of these animals were typed to 20 microsatellite markers using multiplex PCR in order to verify inbreeding coefficient (α) and level of heterozygosity. We found an average heterozygosity of 64% and obtained α=-0.03293 (±0.00573). Our results indicate that the reproductive strategy used was effective because consanguineous breeding was avoided. A continuous genetic program must be carried out in order to obtain better quality primates for biomedical research.

Original languageEnglish (US)
Pages (from-to)183-186
Number of pages4
JournalPrimates
Volume45
Issue number3
DOIs
StatePublished - Jul 2004

Fingerprint

Macaca mulatta
biomedical research
breeding
animals
heterozygosity
inbreeding coefficient
pedigree
monkeys
Primates
microsatellite repeats
genetic variation
DNA
sampling

Keywords

  • Animal management
  • Genetic diversity
  • Inbreeding
  • Macaca mulatta

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Andrade, M. C. R., Penedo, C., Ward, T., Silva, V. F., Bertolini, L. R., Roberts, J. A., ... Cabello, P. H. (2004). Determination of genetic status in a closed colony of rhesus monkeys (Macaca mulatta). Primates, 45(3), 183-186. https://doi.org/10.1007/s10329-004-0084-x

Determination of genetic status in a closed colony of rhesus monkeys (Macaca mulatta). / Andrade, Marcia C Ribeiro; Penedo, Cecilia; Ward, Thea; Silva, Virgilio F.; Bertolini, Luciana R.; Roberts, Jeffrey A; Paulo, Jose Paulo G; Cabello, Pedro H.

In: Primates, Vol. 45, No. 3, 07.2004, p. 183-186.

Research output: Contribution to journalArticle

Andrade, MCR, Penedo, C, Ward, T, Silva, VF, Bertolini, LR, Roberts, JA, Paulo, JPG & Cabello, PH 2004, 'Determination of genetic status in a closed colony of rhesus monkeys (Macaca mulatta)', Primates, vol. 45, no. 3, pp. 183-186. https://doi.org/10.1007/s10329-004-0084-x
Andrade, Marcia C Ribeiro ; Penedo, Cecilia ; Ward, Thea ; Silva, Virgilio F. ; Bertolini, Luciana R. ; Roberts, Jeffrey A ; Paulo, Jose Paulo G ; Cabello, Pedro H. / Determination of genetic status in a closed colony of rhesus monkeys (Macaca mulatta). In: Primates. 2004 ; Vol. 45, No. 3. pp. 183-186.
@article{d3a588f47f3e4d999d4e3b0066307fab,
title = "Determination of genetic status in a closed colony of rhesus monkeys (Macaca mulatta)",
abstract = "The long-term management of breeding colonies requires some measure of genetic diversity in the animal population. For the maintenance of breeding colonies of monkeys used for biomedical research, known pedigrees supply precise data to determine the genetic status of colonies. We present data of genetic analyses in an old closed colony of rhesus macaques (Macaca mulatta) that was established in 1932 with 100 animals. For more than 40 years, the animals were kept on an isolated island and, in 1980, single-male breeding groups were established. A total of 333 DNA samples of these animals were typed to 20 microsatellite markers using multiplex PCR in order to verify inbreeding coefficient (α) and level of heterozygosity. We found an average heterozygosity of 64{\%} and obtained α=-0.03293 (±0.00573). Our results indicate that the reproductive strategy used was effective because consanguineous breeding was avoided. A continuous genetic program must be carried out in order to obtain better quality primates for biomedical research.",
keywords = "Animal management, Genetic diversity, Inbreeding, Macaca mulatta",
author = "Andrade, {Marcia C Ribeiro} and Cecilia Penedo and Thea Ward and Silva, {Virgilio F.} and Bertolini, {Luciana R.} and Roberts, {Jeffrey A} and Paulo, {Jose Paulo G} and Cabello, {Pedro H.}",
year = "2004",
month = "7",
doi = "10.1007/s10329-004-0084-x",
language = "English (US)",
volume = "45",
pages = "183--186",
journal = "Primates",
issn = "0032-8332",
publisher = "Springer Japan",
number = "3",

}

TY - JOUR

T1 - Determination of genetic status in a closed colony of rhesus monkeys (Macaca mulatta)

AU - Andrade, Marcia C Ribeiro

AU - Penedo, Cecilia

AU - Ward, Thea

AU - Silva, Virgilio F.

AU - Bertolini, Luciana R.

AU - Roberts, Jeffrey A

AU - Paulo, Jose Paulo G

AU - Cabello, Pedro H.

PY - 2004/7

Y1 - 2004/7

N2 - The long-term management of breeding colonies requires some measure of genetic diversity in the animal population. For the maintenance of breeding colonies of monkeys used for biomedical research, known pedigrees supply precise data to determine the genetic status of colonies. We present data of genetic analyses in an old closed colony of rhesus macaques (Macaca mulatta) that was established in 1932 with 100 animals. For more than 40 years, the animals were kept on an isolated island and, in 1980, single-male breeding groups were established. A total of 333 DNA samples of these animals were typed to 20 microsatellite markers using multiplex PCR in order to verify inbreeding coefficient (α) and level of heterozygosity. We found an average heterozygosity of 64% and obtained α=-0.03293 (±0.00573). Our results indicate that the reproductive strategy used was effective because consanguineous breeding was avoided. A continuous genetic program must be carried out in order to obtain better quality primates for biomedical research.

AB - The long-term management of breeding colonies requires some measure of genetic diversity in the animal population. For the maintenance of breeding colonies of monkeys used for biomedical research, known pedigrees supply precise data to determine the genetic status of colonies. We present data of genetic analyses in an old closed colony of rhesus macaques (Macaca mulatta) that was established in 1932 with 100 animals. For more than 40 years, the animals were kept on an isolated island and, in 1980, single-male breeding groups were established. A total of 333 DNA samples of these animals were typed to 20 microsatellite markers using multiplex PCR in order to verify inbreeding coefficient (α) and level of heterozygosity. We found an average heterozygosity of 64% and obtained α=-0.03293 (±0.00573). Our results indicate that the reproductive strategy used was effective because consanguineous breeding was avoided. A continuous genetic program must be carried out in order to obtain better quality primates for biomedical research.

KW - Animal management

KW - Genetic diversity

KW - Inbreeding

KW - Macaca mulatta

UR - http://www.scopus.com/inward/record.url?scp=4344580620&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=4344580620&partnerID=8YFLogxK

U2 - 10.1007/s10329-004-0084-x

DO - 10.1007/s10329-004-0084-x

M3 - Article

C2 - 15103562

AN - SCOPUS:4344580620

VL - 45

SP - 183

EP - 186

JO - Primates

JF - Primates

SN - 0032-8332

IS - 3

ER -