Determination of cytotoxicity of nephrotoxins on murine and human kidney cell lines

Eun Kee Park, Sally K. Mak, Dietmar Kültz, Bruce D. Hammock

Research output: Contribution to journalArticle

8 Scopus citations

Abstract

The present study investigates renal inner medullary collecting duct (mIMCD3) cells and human embryonic kidney cells (HEK293) for evaluation of cytotoxicity of nephrotoxic compounds. The 24 h LC50 values for cisplatin, paraquat and ibuprofen in mIMCD3 cells were 135, 155 and 3600 μM, respectively. The 24 h LC50 values for paraquat and ibuprofen in HEK293 cells were 180 and 1000 μM, respectively. Effects of hyperosmolality on cytotoxicity of paraquat were additive in mIMCD3 cells. These data demonstrate that renal hyperosmolality has an additive effect on cytoxicity of paraquat.

Original languageEnglish (US)
Pages (from-to)71-74
Number of pages4
JournalJournal of Environmental Science and Health - Part B Pesticides, Food Contaminants, and Agricultural Wastes
Volume43
Issue number1
DOIs
StatePublished - Jan 2008

Keywords

  • HEK293 cells
  • Hyperosmolality
  • MIMCD3 cells
  • Paraquat

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health
  • Waste Management and Disposal

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