Determination of bovine serum albumin conjugated drugs by immobilization as microarrays and oblique-incidence reflectivity difference microscopy

Yung Shin Sun, Kit Lam, X. D. Zhu

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

Human serum is a mixture of various proteins which may interact with drugs. Therefore, it is of interest to investigate the binding kinetics of pharmaceuticals with their corresponding antibodies in serum. In this article, microarrays and a label-free biosensor were used to study these interactions. Microarrays provide a high-throughput platform for characterizing biomolecular interactions, and the label-free oblique-incidence reflectivity difference biosensor avoids the drawbacks of fluorescence-based methods. The experimental results show that the binding affinities between most of the drugs and their antibodies were reduced in human serum because the bulky proteins block the access to or reduce the stability of the reaction complexes. Therefore, one should be mindful when in vitro or in vivo testing the efficiency of potential drugs and their antibodies.

Original languageEnglish (US)
Pages (from-to)475-485
Number of pages11
JournalInstrumentation Science and Technology
Volume42
Issue number4
DOIs
StatePublished - Jul 4 2014

Keywords

  • drug-antibody binding affinity
  • label-free
  • microarray
  • oblique-incidence reflectivity difference (OI-RD)

ASJC Scopus subject areas

  • Instrumentation
  • Chemical Engineering(all)
  • Environmental Science(all)

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