Determination of antigenic specificity and relationship among Haemophilus pleuropneumoniae serotypes by an indirect hemagglutination test

K. R. Mittal, Robert Higgins, S. Lariviere

Research output: Contribution to journalArticle

29 Scopus citations

Abstract

Serological properties of antigens extracted from strains of Haemophilus pleuropneumoniae belonging to seven different serotypes were investigated. Antisera were prepared in rabbits against Formalin-treated whole cell suspensions as well as autoclaved cell suspensions. Saline and heat extracts and their alcohol precipitate antigens of H. pleuropneumoniae were used in the indirect hemagglutination test. All the antigens used were easily adsorbed directly onto sheep erythrocytes. Saline extract antigen showed maximum type specificity. Heating of the whole cell suspension revealed the cross-reactive minor antigenic determinants. Thus, the heat extract preparations had both serotyping and serodiagnosis of H. pleuropneumoniae infections in pigs.

Original languageEnglish (US)
Pages (from-to)787-790
Number of pages4
JournalJournal of Clinical Microbiology
Volume17
Issue number5
StatePublished - Jan 1 1983
Externally publishedYes

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Fingerprint Dive into the research topics of 'Determination of antigenic specificity and relationship among Haemophilus pleuropneumoniae serotypes by an indirect hemagglutination test'. Together they form a unique fingerprint.

  • Cite this