Determination of acepromazine, ketamine, medetomidine, and xylazine in serum

Multi-residue screening by liquid chromatography-mass spectrometry

Irina Rudik Miksa, Margaret R. Cummings, Robert H Poppenga

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A large variety of drugs are administered to large and small animals by veterinary clinicians for sedation, anesthesia, muscle relaxation, and analgesia. The present paper reports a simple and rapid multi-residue detection and quantitation method for four chemically different drugs: medetomidine, xylazine, ketamine, and acepromazine. Chromatographic separation was carried out on a liquid chromatography-mass spectrometry instrument with a C18-reversed-phase column. Fragmentation patterns were determined with atmospheric pressure chemical ionization mass spectrometry set to operate in a positive selective ion monitoring mode. The method was determined to be linear over the range of concentrations tested (2.0-100.0 ng/mL). Accuracy, precision, and specificity were evaluated and the method was determined to be applicable to detection of medetomidine, xylazine, ketamine, and acepromazine in serum samples of multiple animal species (canine, equine, and bovine). Matrix limits of quantitation were determined to be 5.0 ng/mL for all four analytes, and recoveries ranged between 82.0 and 118%, with a 3.0-18.3% relative standard deviation.

Original languageEnglish (US)
Pages (from-to)544-551
Number of pages8
JournalJournal of Analytical Toxicology
Volume29
Issue number6
StatePublished - Sep 2005
Externally publishedYes

Fingerprint

Acepromazine
Medetomidine
Xylazine
Liquid chromatography
Ketamine
Liquid Chromatography
Mass spectrometry
liquid chromatography
serum
Mass Spectrometry
Screening
Animals
mass spectrometry
drug
Serum
Pharmaceutical Preparations
Atmospheric pressure
Ionization
Muscle
Atmospheric Pressure

ASJC Scopus subject areas

  • Analytical Chemistry
  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

Determination of acepromazine, ketamine, medetomidine, and xylazine in serum : Multi-residue screening by liquid chromatography-mass spectrometry. / Miksa, Irina Rudik; Cummings, Margaret R.; Poppenga, Robert H.

In: Journal of Analytical Toxicology, Vol. 29, No. 6, 09.2005, p. 544-551.

Research output: Contribution to journalArticle

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