Abstract
A large variety of drugs are administered to large and small animals by veterinary clinicians for sedation, anesthesia, muscle relaxation, and analgesia. The present paper reports a simple and rapid multi-residue detection and quantitation method for four chemically different drugs: medetomidine, xylazine, ketamine, and acepromazine. Chromatographic separation was carried out on a liquid chromatography-mass spectrometry instrument with a C18-reversed-phase column. Fragmentation patterns were determined with atmospheric pressure chemical ionization mass spectrometry set to operate in a positive selective ion monitoring mode. The method was determined to be linear over the range of concentrations tested (2.0-100.0 ng/mL). Accuracy, precision, and specificity were evaluated and the method was determined to be applicable to detection of medetomidine, xylazine, ketamine, and acepromazine in serum samples of multiple animal species (canine, equine, and bovine). Matrix limits of quantitation were determined to be 5.0 ng/mL for all four analytes, and recoveries ranged between 82.0 and 118%, with a 3.0-18.3% relative standard deviation.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 544-551 |
| Number of pages | 8 |
| Journal | Journal of Analytical Toxicology |
| Volume | 29 |
| Issue number | 6 |
| State | Published - Sep 2005 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Analytical Chemistry
- Toxicology
- Health, Toxicology and Mutagenesis
Cite this
Determination of acepromazine, ketamine, medetomidine, and xylazine in serum : Multi-residue screening by liquid chromatography-mass spectrometry. / Miksa, Irina Rudik; Cummings, Margaret R.; Poppenga, Robert H.
In: Journal of Analytical Toxicology, Vol. 29, No. 6, 09.2005, p. 544-551.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Determination of acepromazine, ketamine, medetomidine, and xylazine in serum
T2 - Multi-residue screening by liquid chromatography-mass spectrometry
AU - Miksa, Irina Rudik
AU - Cummings, Margaret R.
AU - Poppenga, Robert H
PY - 2005/9
Y1 - 2005/9
N2 - A large variety of drugs are administered to large and small animals by veterinary clinicians for sedation, anesthesia, muscle relaxation, and analgesia. The present paper reports a simple and rapid multi-residue detection and quantitation method for four chemically different drugs: medetomidine, xylazine, ketamine, and acepromazine. Chromatographic separation was carried out on a liquid chromatography-mass spectrometry instrument with a C18-reversed-phase column. Fragmentation patterns were determined with atmospheric pressure chemical ionization mass spectrometry set to operate in a positive selective ion monitoring mode. The method was determined to be linear over the range of concentrations tested (2.0-100.0 ng/mL). Accuracy, precision, and specificity were evaluated and the method was determined to be applicable to detection of medetomidine, xylazine, ketamine, and acepromazine in serum samples of multiple animal species (canine, equine, and bovine). Matrix limits of quantitation were determined to be 5.0 ng/mL for all four analytes, and recoveries ranged between 82.0 and 118%, with a 3.0-18.3% relative standard deviation.
AB - A large variety of drugs are administered to large and small animals by veterinary clinicians for sedation, anesthesia, muscle relaxation, and analgesia. The present paper reports a simple and rapid multi-residue detection and quantitation method for four chemically different drugs: medetomidine, xylazine, ketamine, and acepromazine. Chromatographic separation was carried out on a liquid chromatography-mass spectrometry instrument with a C18-reversed-phase column. Fragmentation patterns were determined with atmospheric pressure chemical ionization mass spectrometry set to operate in a positive selective ion monitoring mode. The method was determined to be linear over the range of concentrations tested (2.0-100.0 ng/mL). Accuracy, precision, and specificity were evaluated and the method was determined to be applicable to detection of medetomidine, xylazine, ketamine, and acepromazine in serum samples of multiple animal species (canine, equine, and bovine). Matrix limits of quantitation were determined to be 5.0 ng/mL for all four analytes, and recoveries ranged between 82.0 and 118%, with a 3.0-18.3% relative standard deviation.
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M3 - Article
VL - 29
SP - 544
EP - 551
JO - Journal of Analytical Toxicology
JF - Journal of Analytical Toxicology
SN - 0146-4760
IS - 6
ER -