Detection of xenoestrogens in serum after immunoprecipitation of endogenous steroidal estrogens

Kala Natarajan, James W. Overstreet, Jane M. Rogers, Michael S. Denison, Jiangang Chen, Peter N. Lohstroh, Daniel S. McConnell, Bill L. Lasley

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

In this article we report a simple and efficient method for detecting nonsteroidal estrogens in a biologic sample. This method uses polyclonal antibodies to estradiol (E2) to immunoprecipitate these major biologically active steroidal estrogens, leaving behind the nonsteroidal estrogens, which are then detected in a cell-based transcriptional activation bioassay for estrogen receptor agonist. The immunoprecipitation method effeciently removed 99% of radiolabeled E2 and estrone (E1) from human serum. In experiments in which supraphysiologic concentrations of E2 and E1 to human serum, all of the immunoreactive estrogens were still removed by the immunoprecipitation protocol. We carried out an in vivo validation study of this method in which we treated female macaques with the xenoestrogen nonylphenol (NP), during the late follicular phase of the menstrual cycle. We used blood samples collected before and after treatment to evaluate and characterize endogenous and exogenous serum estrogens. An immunoassay for E2 did not detect the NP in treated monkeys. The cell-based bioassay also did not detect the estrogenic activity of NP because of its saturation by the endogenous serum steroidal estrogens. However, when steroidal estrogens were removed by immunoprecipitation, we detected the estrogenic activity of NP in the bioassay. Thus, this approach is appropriate for detecting exogenous, nonsteroidal estrogens in serum samples.

Original languageEnglish (US)
Pages (from-to)791-795
Number of pages5
JournalEnvironmental Health Perspectives
Volume110
Issue number8
StatePublished - 2002

Fingerprint

Immunoprecipitation
serum
Estrogens
Non-Steroidal Estrogens
Bioassay
bioassay
Serum
Biological Assay
immunoassay
Follicular Phase
Estrone
Validation Studies
antibody
Macaca
Immunoassay
Transcriptional Activation
blood
Haplorhini
detection
Estradiol

Keywords

  • Cell bioassay
  • Endocrine disruptors
  • Nonylphenol
  • Steroidal estrogens
  • Xenoestrogens

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health

Cite this

Natarajan, K., Overstreet, J. W., Rogers, J. M., Denison, M. S., Chen, J., Lohstroh, P. N., ... Lasley, B. L. (2002). Detection of xenoestrogens in serum after immunoprecipitation of endogenous steroidal estrogens. Environmental Health Perspectives, 110(8), 791-795.

Detection of xenoestrogens in serum after immunoprecipitation of endogenous steroidal estrogens. / Natarajan, Kala; Overstreet, James W.; Rogers, Jane M.; Denison, Michael S.; Chen, Jiangang; Lohstroh, Peter N.; McConnell, Daniel S.; Lasley, Bill L.

In: Environmental Health Perspectives, Vol. 110, No. 8, 2002, p. 791-795.

Research output: Contribution to journalArticle

Natarajan, K, Overstreet, JW, Rogers, JM, Denison, MS, Chen, J, Lohstroh, PN, McConnell, DS & Lasley, BL 2002, 'Detection of xenoestrogens in serum after immunoprecipitation of endogenous steroidal estrogens', Environmental Health Perspectives, vol. 110, no. 8, pp. 791-795.
Natarajan K, Overstreet JW, Rogers JM, Denison MS, Chen J, Lohstroh PN et al. Detection of xenoestrogens in serum after immunoprecipitation of endogenous steroidal estrogens. Environmental Health Perspectives. 2002;110(8):791-795.
Natarajan, Kala ; Overstreet, James W. ; Rogers, Jane M. ; Denison, Michael S. ; Chen, Jiangang ; Lohstroh, Peter N. ; McConnell, Daniel S. ; Lasley, Bill L. / Detection of xenoestrogens in serum after immunoprecipitation of endogenous steroidal estrogens. In: Environmental Health Perspectives. 2002 ; Vol. 110, No. 8. pp. 791-795.
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