Detection of viral antigens in bluetongue virus-infected ovine tissues, using the peroxidase-antiperoxidase technique.

J. M. Cherrington, H. W. Ghalib, M. M. Sawyer, Bennie Osburn

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

An indirect immunoperoxidase procedure was developed to detect viral antigens in bluetongue virus (BTV)-infected tissues. Embryonating chicken eggs were infected with BTV serotypes 10, 11, 13, or 17, and the chorioallantoic membranes were subsequently fixed in formalin and embedded in paraffin. The peroxidase-antiperoxidase (PAP) system was used to examine the infected membranes for the presence of viral antigens. Sheep antisera raised against BTV serotypes 10, 11, 13, and 17 served as the primary antibodies in the PAP procedure. Specific staining was observed when each of these antisera was applied to membranes expressing antigens of homologous and heterologous BTV serotypes. The PAP method was rapid, reliable, and specific in its detection of BTV.

Original languageEnglish (US)
Pages (from-to)2356-2359
Number of pages4
JournalAmerican Journal of Veterinary Research
Volume46
Issue number11
StatePublished - Nov 1 1985
Externally publishedYes

Fingerprint

Bluetongue virus
Viral Antigens
viral antigens
Peroxidase
Sheep
peroxidase
sheep
serotypes
antiserum
Immune Sera
Heterophile Antigens
Chorioallantoic Membrane
chorioallantoic membrane
methodology
Membranes
chicken eggs
formalin
Paraffin
alkanes
Eggs

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Detection of viral antigens in bluetongue virus-infected ovine tissues, using the peroxidase-antiperoxidase technique. / Cherrington, J. M.; Ghalib, H. W.; Sawyer, M. M.; Osburn, Bennie.

In: American Journal of Veterinary Research, Vol. 46, No. 11, 01.11.1985, p. 2356-2359.

Research output: Contribution to journalArticle

Cherrington, J. M. ; Ghalib, H. W. ; Sawyer, M. M. ; Osburn, Bennie. / Detection of viral antigens in bluetongue virus-infected ovine tissues, using the peroxidase-antiperoxidase technique. In: American Journal of Veterinary Research. 1985 ; Vol. 46, No. 11. pp. 2356-2359.
@article{d64e686123d74b43b91b67a6944a1cb2,
title = "Detection of viral antigens in bluetongue virus-infected ovine tissues, using the peroxidase-antiperoxidase technique.",
abstract = "An indirect immunoperoxidase procedure was developed to detect viral antigens in bluetongue virus (BTV)-infected tissues. Embryonating chicken eggs were infected with BTV serotypes 10, 11, 13, or 17, and the chorioallantoic membranes were subsequently fixed in formalin and embedded in paraffin. The peroxidase-antiperoxidase (PAP) system was used to examine the infected membranes for the presence of viral antigens. Sheep antisera raised against BTV serotypes 10, 11, 13, and 17 served as the primary antibodies in the PAP procedure. Specific staining was observed when each of these antisera was applied to membranes expressing antigens of homologous and heterologous BTV serotypes. The PAP method was rapid, reliable, and specific in its detection of BTV.",
author = "Cherrington, {J. M.} and Ghalib, {H. W.} and Sawyer, {M. M.} and Bennie Osburn",
year = "1985",
month = "11",
day = "1",
language = "English (US)",
volume = "46",
pages = "2356--2359",
journal = "American Journal of Veterinary Research",
issn = "0002-9645",
publisher = "American Veterinary Medical Association",
number = "11",

}

TY - JOUR

T1 - Detection of viral antigens in bluetongue virus-infected ovine tissues, using the peroxidase-antiperoxidase technique.

AU - Cherrington, J. M.

AU - Ghalib, H. W.

AU - Sawyer, M. M.

AU - Osburn, Bennie

PY - 1985/11/1

Y1 - 1985/11/1

N2 - An indirect immunoperoxidase procedure was developed to detect viral antigens in bluetongue virus (BTV)-infected tissues. Embryonating chicken eggs were infected with BTV serotypes 10, 11, 13, or 17, and the chorioallantoic membranes were subsequently fixed in formalin and embedded in paraffin. The peroxidase-antiperoxidase (PAP) system was used to examine the infected membranes for the presence of viral antigens. Sheep antisera raised against BTV serotypes 10, 11, 13, and 17 served as the primary antibodies in the PAP procedure. Specific staining was observed when each of these antisera was applied to membranes expressing antigens of homologous and heterologous BTV serotypes. The PAP method was rapid, reliable, and specific in its detection of BTV.

AB - An indirect immunoperoxidase procedure was developed to detect viral antigens in bluetongue virus (BTV)-infected tissues. Embryonating chicken eggs were infected with BTV serotypes 10, 11, 13, or 17, and the chorioallantoic membranes were subsequently fixed in formalin and embedded in paraffin. The peroxidase-antiperoxidase (PAP) system was used to examine the infected membranes for the presence of viral antigens. Sheep antisera raised against BTV serotypes 10, 11, 13, and 17 served as the primary antibodies in the PAP procedure. Specific staining was observed when each of these antisera was applied to membranes expressing antigens of homologous and heterologous BTV serotypes. The PAP method was rapid, reliable, and specific in its detection of BTV.

UR - http://www.scopus.com/inward/record.url?scp=0022156764&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022156764&partnerID=8YFLogxK

M3 - Article

C2 - 3000230

AN - SCOPUS:0022156764

VL - 46

SP - 2356

EP - 2359

JO - American Journal of Veterinary Research

JF - American Journal of Veterinary Research

SN - 0002-9645

IS - 11

ER -