Detection of thrombin by aptamerbased surface enhanced resonance raman spectroscopy

Hansang Cho, Brian R. Baker, Sebastian Wachsmann-Hogiu, Cynthia Pagba, Ted Laurence, Stephen M. Lane, Luke P. Lee, Jeffrey B H Tok

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

This paper reports a selective and sensitive optical detection method using aptamer- based surface enhanced resonance Raman spectroscopy (SERRS). Here we successfully demonstrate thrombin detection down to 100 pM by observing SERRS signal change upon binding. Furthermore, no binding was observed from nonbinding protein or non-thrombin binding aptamer. The aptamer is sufficiently stable to detect 1 nM thrombin in the presence of 10% serum. The proposed detection method may be further implemented for multiplexed detection using different aptamerbased Raman probe complexes.

Original languageEnglish (US)
Title of host publication12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference
PublisherChemical and Biological Microsystems Society
Pages290-292
Number of pages3
StatePublished - 2008
Event12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008 - San Diego, CA, United States
Duration: Oct 12 2008Oct 16 2008

Other

Other12th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2008
CountryUnited States
CitySan Diego, CA
Period10/12/0810/16/08

Keywords

  • Aptamer
  • Oligonucleotide
  • Surface-enhanced resonance raman spectroscopy
  • Thrombin detection

ASJC Scopus subject areas

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Cho, H., Baker, B. R., Wachsmann-Hogiu, S., Pagba, C., Laurence, T., Lane, S. M., Lee, L. P., & Tok, J. B. H. (2008). Detection of thrombin by aptamerbased surface enhanced resonance raman spectroscopy. In 12th International Conference on Miniaturized Systems for Chemistry and Life Sciences - The Proceedings of MicroTAS 2008 Conference (pp. 290-292). Chemical and Biological Microsystems Society.