Detection of SIV DNA in rhesus macaque polymorphonuclear neutrophils

Steffney E. Rought; Gang Li; Kenneth M. Lam; Linda F. Chuang; Bennie Osburn; Ronald Y. Chuang

Detection of SIV DNA in rhesus macaque polymorphonuclear neutrophils

Steffney E. Rought, Gang Li, Kenneth M. Lam, Linda F. Chuang, Bennie Osburn, Ronald Y. Chuang

UC Davis School of Medicine

Research output: Contribution to journal › Article

4 Scopus citations

Abstract

The extent of infection of monkey polymorphonuclear neutrophils (PMN) by simian immunodeficiency virus( SIV) has not yet been determined. Using the polymerase chain reaction (PCR) technique, we detected the presence of SIVmac239 DNA in rhesus macaque-derived PMN after 24 hrs of in vitro incubation of the cells with SIVmac239. Infection by SIVmac239-induced PMN intracellular alterations were correlated with an accelerated decrease in PMN viability over a period of 120 hrs compared to non-infected PMN. Evidence of chromatin condensation characteristic of programmed cell death (apoptosis) was also observed in SIVmac239-infected PMN. The results of this study provide a mechanism for the reduced chemotaxis/phagocytosis activities of PMN of SIVmac239-infected macaques and suggest that PMN is one of the target cells for SIVmac239 infection.

Original language	English (US)
Pages (from-to)	189-193
Number of pages	5
Journal	In Vivo
Volume	13
Issue number	2
State	Published - Mar 1 1999

Keywords

AIDS
Apoptosis
Neutrophils
Rhesus
SIV

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)
Pharmacology

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title = "Detection of SIV DNA in rhesus macaque polymorphonuclear neutrophils",

abstract = "The extent of infection of monkey polymorphonuclear neutrophils (PMN) by simian immunodeficiency virus( SIV) has not yet been determined. Using the polymerase chain reaction (PCR) technique, we detected the presence of SIVmac239 DNA in rhesus macaque-derived PMN after 24 hrs of in vitro incubation of the cells with SIVmac239. Infection by SIVmac239-induced PMN intracellular alterations were correlated with an accelerated decrease in PMN viability over a period of 120 hrs compared to non-infected PMN. Evidence of chromatin condensation characteristic of programmed cell death (apoptosis) was also observed in SIVmac239-infected PMN. The results of this study provide a mechanism for the reduced chemotaxis/phagocytosis activities of PMN of SIVmac239-infected macaques and suggest that PMN is one of the target cells for SIVmac239 infection.",

keywords = "AIDS, Apoptosis, Neutrophils, Rhesus, SIV",

author = "Rought, {Steffney E.} and Gang Li and Lam, {Kenneth M.} and Chuang, {Linda F.} and Bennie Osburn and Chuang, {Ronald Y.}",

year = "1999",

month = mar,

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language = "English (US)",

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T1 - Detection of SIV DNA in rhesus macaque polymorphonuclear neutrophils

AU - Rought, Steffney E.

AU - Li, Gang

AU - Lam, Kenneth M.

AU - Chuang, Linda F.

AU - Osburn, Bennie

AU - Chuang, Ronald Y.

PY - 1999/3/1

Y1 - 1999/3/1

N2 - The extent of infection of monkey polymorphonuclear neutrophils (PMN) by simian immunodeficiency virus( SIV) has not yet been determined. Using the polymerase chain reaction (PCR) technique, we detected the presence of SIVmac239 DNA in rhesus macaque-derived PMN after 24 hrs of in vitro incubation of the cells with SIVmac239. Infection by SIVmac239-induced PMN intracellular alterations were correlated with an accelerated decrease in PMN viability over a period of 120 hrs compared to non-infected PMN. Evidence of chromatin condensation characteristic of programmed cell death (apoptosis) was also observed in SIVmac239-infected PMN. The results of this study provide a mechanism for the reduced chemotaxis/phagocytosis activities of PMN of SIVmac239-infected macaques and suggest that PMN is one of the target cells for SIVmac239 infection.

AB - The extent of infection of monkey polymorphonuclear neutrophils (PMN) by simian immunodeficiency virus( SIV) has not yet been determined. Using the polymerase chain reaction (PCR) technique, we detected the presence of SIVmac239 DNA in rhesus macaque-derived PMN after 24 hrs of in vitro incubation of the cells with SIVmac239. Infection by SIVmac239-induced PMN intracellular alterations were correlated with an accelerated decrease in PMN viability over a period of 120 hrs compared to non-infected PMN. Evidence of chromatin condensation characteristic of programmed cell death (apoptosis) was also observed in SIVmac239-infected PMN. The results of this study provide a mechanism for the reduced chemotaxis/phagocytosis activities of PMN of SIVmac239-infected macaques and suggest that PMN is one of the target cells for SIVmac239 infection.

KW - AIDS

KW - Apoptosis

KW - Neutrophils

KW - Rhesus

KW - SIV

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