Abstract
The extent of infection of monkey polymorphonuclear neutrophils (PMN) by simian immunodeficiency virus( SIV) has not yet been determined. Using the polymerase chain reaction (PCR) technique, we detected the presence of SIVmac239 DNA in rhesus macaque-derived PMN after 24 hrs of in vitro incubation of the cells with SIVmac239. Infection by SIVmac239-induced PMN intracellular alterations were correlated with an accelerated decrease in PMN viability over a period of 120 hrs compared to non-infected PMN. Evidence of chromatin condensation characteristic of programmed cell death (apoptosis) was also observed in SIVmac239-infected PMN. The results of this study provide a mechanism for the reduced chemotaxis/phagocytosis activities of PMN of SIVmac239-infected macaques and suggest that PMN is one of the target cells for SIVmac239 infection.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 189-193 |
| Number of pages | 5 |
| Journal | In Vivo |
| Volume | 13 |
| Issue number | 2 |
| State | Published - Mar 1 1999 |
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Keywords
- AIDS
- Apoptosis
- Neutrophils
- Rhesus
- SIV
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
- Pharmacology
Cite this
Detection of SIV DNA in rhesus macaque polymorphonuclear neutrophils. / Rought, Steffney E.; Li, Gang; Lam, Kenneth M.; Chuang, Linda F.; Osburn, Bennie; Chuang, Ronald Y.
In: In Vivo, Vol. 13, No. 2, 01.03.1999, p. 189-193.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of SIV DNA in rhesus macaque polymorphonuclear neutrophils
AU - Rought, Steffney E.
AU - Li, Gang
AU - Lam, Kenneth M.
AU - Chuang, Linda F.
AU - Osburn, Bennie
AU - Chuang, Ronald Y.
PY - 1999/3/1
Y1 - 1999/3/1
N2 - The extent of infection of monkey polymorphonuclear neutrophils (PMN) by simian immunodeficiency virus( SIV) has not yet been determined. Using the polymerase chain reaction (PCR) technique, we detected the presence of SIVmac239 DNA in rhesus macaque-derived PMN after 24 hrs of in vitro incubation of the cells with SIVmac239. Infection by SIVmac239-induced PMN intracellular alterations were correlated with an accelerated decrease in PMN viability over a period of 120 hrs compared to non-infected PMN. Evidence of chromatin condensation characteristic of programmed cell death (apoptosis) was also observed in SIVmac239-infected PMN. The results of this study provide a mechanism for the reduced chemotaxis/phagocytosis activities of PMN of SIVmac239-infected macaques and suggest that PMN is one of the target cells for SIVmac239 infection.
AB - The extent of infection of monkey polymorphonuclear neutrophils (PMN) by simian immunodeficiency virus( SIV) has not yet been determined. Using the polymerase chain reaction (PCR) technique, we detected the presence of SIVmac239 DNA in rhesus macaque-derived PMN after 24 hrs of in vitro incubation of the cells with SIVmac239. Infection by SIVmac239-induced PMN intracellular alterations were correlated with an accelerated decrease in PMN viability over a period of 120 hrs compared to non-infected PMN. Evidence of chromatin condensation characteristic of programmed cell death (apoptosis) was also observed in SIVmac239-infected PMN. The results of this study provide a mechanism for the reduced chemotaxis/phagocytosis activities of PMN of SIVmac239-infected macaques and suggest that PMN is one of the target cells for SIVmac239 infection.
KW - AIDS
KW - Apoptosis
KW - Neutrophils
KW - Rhesus
KW - SIV
UR - http://www.scopus.com/inward/record.url?scp=0032953775&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0032953775&partnerID=8YFLogxK
M3 - Article
C2 - 10363177
AN - SCOPUS:0032953775
VL - 13
SP - 189
EP - 193
JO - In Vivo
JF - In Vivo
SN - 0258-851X
IS - 2
ER -