Detection of SIV DNA in rhesus macaque polymorphonuclear neutrophils

Steffney E. Rought, Gang Li, Kenneth M. Lam, Linda F. Chuang, Bennie Osburn, Ronald Y. Chuang

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

The extent of infection of monkey polymorphonuclear neutrophils (PMN) by simian immunodeficiency virus( SIV) has not yet been determined. Using the polymerase chain reaction (PCR) technique, we detected the presence of SIVmac239 DNA in rhesus macaque-derived PMN after 24 hrs of in vitro incubation of the cells with SIVmac239. Infection by SIVmac239-induced PMN intracellular alterations were correlated with an accelerated decrease in PMN viability over a period of 120 hrs compared to non-infected PMN. Evidence of chromatin condensation characteristic of programmed cell death (apoptosis) was also observed in SIVmac239-infected PMN. The results of this study provide a mechanism for the reduced chemotaxis/phagocytosis activities of PMN of SIVmac239-infected macaques and suggest that PMN is one of the target cells for SIVmac239 infection.

Original languageEnglish (US)
Pages (from-to)189-193
Number of pages5
JournalIn Vivo
Volume13
Issue number2
StatePublished - Mar 1 1999

Keywords

  • AIDS
  • Apoptosis
  • Neutrophils
  • Rhesus
  • SIV

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology

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