Detection of serum nitrite and nitrate in primary biliary cirrhosis: Possible role of nitric oxide in bile duct injury

Atsushi Hokari, Mikio Zeniya, Hiroyasu Esumi, Tomonobu Kawabe, M. Eric Gershwin, Gotaro Toda

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Background: The role of nitric oxide synthase (NOS) in autoimmune disease is gaining increased attention because of the relationships between NOS activity and T-lymphocyte subpopulations and, in particular, the influence of NO on cytokine production by Th1 versus Th2 cells. In addition, there is evidence that both the liver and infiltrating hepatic T cells have inducible NOS-2 activity. Methods: We studied serum levels of nitrite (NO2 -) and nitrate (NO3 -) in groups of patients with liver disease secondary to hepatitis B, hepatitis C, autoimmune hepatitis and primary biliary cirrhosis (PBC). Simultaneously, in a nested subpopulation, we studied the liver expression of NOS-2. Results: Interestingly, there was a significant elevation both of nitrite and of nitrate in patients with PBC but not other liver diseases. Despite such increments, there was no correlation of the levels of nitrite and nitrate with sera levels of tumor necrosis factor-α, interferon-γ, alanine aminotransferase, total bilirubin, alkaline phosphatase, gamma-glutamyl transpeptidase, platelet count, IgG, IgM, antimitochondrial antibodies or prothrombin time. These data were extended by demonstrating the expression of NOS-2 by immunohistochemistry in 13/14 patients with PBC, including in 9/14 patient hepatocyte populations and 4/14 bile duct cells. In contrast, NOS-2 expression was noted in hepatitis B and hepatitis C, but only found within mononuclear cells. Conclusion: Our data suggest that NO produced through NOS-2 may play a role in the pathogenesis of bile duct injury in some PBC patients.

Original languageEnglish (US)
Pages (from-to)308-315
Number of pages8
JournalJournal of Gastroenterology and Hepatology (Australia)
Volume17
Issue number3
DOIs
StatePublished - 2002

Fingerprint

Biliary Liver Cirrhosis
Nitrites
Bile Ducts
Nitric Oxide Synthase
Nitrates
Nitric Oxide
Wounds and Injuries
Serum
Hepatitis C
Hepatitis B
Liver Diseases
Hepatocytes
T-Lymphocytes
Autoimmune Hepatitis
Th2 Cells
gamma-Glutamyltransferase
Liver
Prothrombin Time
Lymphocyte Subsets
Nitric Oxide Synthase Type II

Keywords

  • Nitrate
  • Nitric oxide
  • Nitric oxide synthase
  • Nitrite
  • Primary biliary cirrhosis

ASJC Scopus subject areas

  • Gastroenterology
  • Hepatology

Cite this

Detection of serum nitrite and nitrate in primary biliary cirrhosis : Possible role of nitric oxide in bile duct injury. / Hokari, Atsushi; Zeniya, Mikio; Esumi, Hiroyasu; Kawabe, Tomonobu; Gershwin, M. Eric; Toda, Gotaro.

In: Journal of Gastroenterology and Hepatology (Australia), Vol. 17, No. 3, 2002, p. 308-315.

Research output: Contribution to journalArticle

Hokari, Atsushi ; Zeniya, Mikio ; Esumi, Hiroyasu ; Kawabe, Tomonobu ; Gershwin, M. Eric ; Toda, Gotaro. / Detection of serum nitrite and nitrate in primary biliary cirrhosis : Possible role of nitric oxide in bile duct injury. In: Journal of Gastroenterology and Hepatology (Australia). 2002 ; Vol. 17, No. 3. pp. 308-315.
@article{239088b7149842cb886b6e6fcfddfcfa,
title = "Detection of serum nitrite and nitrate in primary biliary cirrhosis: Possible role of nitric oxide in bile duct injury",
abstract = "Background: The role of nitric oxide synthase (NOS) in autoimmune disease is gaining increased attention because of the relationships between NOS activity and T-lymphocyte subpopulations and, in particular, the influence of NO on cytokine production by Th1 versus Th2 cells. In addition, there is evidence that both the liver and infiltrating hepatic T cells have inducible NOS-2 activity. Methods: We studied serum levels of nitrite (NO2 -) and nitrate (NO3 -) in groups of patients with liver disease secondary to hepatitis B, hepatitis C, autoimmune hepatitis and primary biliary cirrhosis (PBC). Simultaneously, in a nested subpopulation, we studied the liver expression of NOS-2. Results: Interestingly, there was a significant elevation both of nitrite and of nitrate in patients with PBC but not other liver diseases. Despite such increments, there was no correlation of the levels of nitrite and nitrate with sera levels of tumor necrosis factor-α, interferon-γ, alanine aminotransferase, total bilirubin, alkaline phosphatase, gamma-glutamyl transpeptidase, platelet count, IgG, IgM, antimitochondrial antibodies or prothrombin time. These data were extended by demonstrating the expression of NOS-2 by immunohistochemistry in 13/14 patients with PBC, including in 9/14 patient hepatocyte populations and 4/14 bile duct cells. In contrast, NOS-2 expression was noted in hepatitis B and hepatitis C, but only found within mononuclear cells. Conclusion: Our data suggest that NO produced through NOS-2 may play a role in the pathogenesis of bile duct injury in some PBC patients.",
keywords = "Nitrate, Nitric oxide, Nitric oxide synthase, Nitrite, Primary biliary cirrhosis",
author = "Atsushi Hokari and Mikio Zeniya and Hiroyasu Esumi and Tomonobu Kawabe and Gershwin, {M. Eric} and Gotaro Toda",
year = "2002",
doi = "10.1046/j.1440-1746.2002.02689.x",
language = "English (US)",
volume = "17",
pages = "308--315",
journal = "Journal of Gastroenterology and Hepatology (Australia)",
issn = "0815-9319",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - Detection of serum nitrite and nitrate in primary biliary cirrhosis

T2 - Possible role of nitric oxide in bile duct injury

AU - Hokari, Atsushi

AU - Zeniya, Mikio

AU - Esumi, Hiroyasu

AU - Kawabe, Tomonobu

AU - Gershwin, M. Eric

AU - Toda, Gotaro

PY - 2002

Y1 - 2002

N2 - Background: The role of nitric oxide synthase (NOS) in autoimmune disease is gaining increased attention because of the relationships between NOS activity and T-lymphocyte subpopulations and, in particular, the influence of NO on cytokine production by Th1 versus Th2 cells. In addition, there is evidence that both the liver and infiltrating hepatic T cells have inducible NOS-2 activity. Methods: We studied serum levels of nitrite (NO2 -) and nitrate (NO3 -) in groups of patients with liver disease secondary to hepatitis B, hepatitis C, autoimmune hepatitis and primary biliary cirrhosis (PBC). Simultaneously, in a nested subpopulation, we studied the liver expression of NOS-2. Results: Interestingly, there was a significant elevation both of nitrite and of nitrate in patients with PBC but not other liver diseases. Despite such increments, there was no correlation of the levels of nitrite and nitrate with sera levels of tumor necrosis factor-α, interferon-γ, alanine aminotransferase, total bilirubin, alkaline phosphatase, gamma-glutamyl transpeptidase, platelet count, IgG, IgM, antimitochondrial antibodies or prothrombin time. These data were extended by demonstrating the expression of NOS-2 by immunohistochemistry in 13/14 patients with PBC, including in 9/14 patient hepatocyte populations and 4/14 bile duct cells. In contrast, NOS-2 expression was noted in hepatitis B and hepatitis C, but only found within mononuclear cells. Conclusion: Our data suggest that NO produced through NOS-2 may play a role in the pathogenesis of bile duct injury in some PBC patients.

AB - Background: The role of nitric oxide synthase (NOS) in autoimmune disease is gaining increased attention because of the relationships between NOS activity and T-lymphocyte subpopulations and, in particular, the influence of NO on cytokine production by Th1 versus Th2 cells. In addition, there is evidence that both the liver and infiltrating hepatic T cells have inducible NOS-2 activity. Methods: We studied serum levels of nitrite (NO2 -) and nitrate (NO3 -) in groups of patients with liver disease secondary to hepatitis B, hepatitis C, autoimmune hepatitis and primary biliary cirrhosis (PBC). Simultaneously, in a nested subpopulation, we studied the liver expression of NOS-2. Results: Interestingly, there was a significant elevation both of nitrite and of nitrate in patients with PBC but not other liver diseases. Despite such increments, there was no correlation of the levels of nitrite and nitrate with sera levels of tumor necrosis factor-α, interferon-γ, alanine aminotransferase, total bilirubin, alkaline phosphatase, gamma-glutamyl transpeptidase, platelet count, IgG, IgM, antimitochondrial antibodies or prothrombin time. These data were extended by demonstrating the expression of NOS-2 by immunohistochemistry in 13/14 patients with PBC, including in 9/14 patient hepatocyte populations and 4/14 bile duct cells. In contrast, NOS-2 expression was noted in hepatitis B and hepatitis C, but only found within mononuclear cells. Conclusion: Our data suggest that NO produced through NOS-2 may play a role in the pathogenesis of bile duct injury in some PBC patients.

KW - Nitrate

KW - Nitric oxide

KW - Nitric oxide synthase

KW - Nitrite

KW - Primary biliary cirrhosis

UR - http://www.scopus.com/inward/record.url?scp=0036097310&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036097310&partnerID=8YFLogxK

U2 - 10.1046/j.1440-1746.2002.02689.x

DO - 10.1046/j.1440-1746.2002.02689.x

M3 - Article

C2 - 11982702

AN - SCOPUS:0036097310

VL - 17

SP - 308

EP - 315

JO - Journal of Gastroenterology and Hepatology (Australia)

JF - Journal of Gastroenterology and Hepatology (Australia)

SN - 0815-9319

IS - 3

ER -