Detection of reticuloendotheliosis virus by immunohistochemistry and in situ hybridization in experimentally infected Japanese quail embryos and archived formalin-fixed and paraffin-embedded tumours

Vitor L S L Santos, Susan M. Williams, Guillermo Zavala, Taylor Barbosa, Jian Zhang, Sunny Cheng, H L Shivaprasad, Scott Hafner, Aly Fadly, Renato L. Santos, Corrie C. Brown

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Abstract

Reticuloendotheliosis virus (REV) infection can result in immunosuppression, a runting syndrome, high mortality, acute reticulum cell neoplasia, or T-cell and/or B-cell lymphomas, in a variety of domestic and wild birds. Histopathological changes in REV infection are not sufficient to differentiate it from avian lymphoid leukosis and Marek's disease, and currently there are no available in situ diagnostic methods for detection of active REV presence in pathologic specimens. To develop immunohistochemistry and in situ hybridization assays for detection of REV active infections, experimentally inoculated Japanese quail embryos, and archived formalin-fixed paraffin-embedded tissues from natural and experimental reticuloendotheliosis cases in chickens and turkeys, were examined. The in situ hybridization and immunohistochemistry assays proved to be efficient for the detection of several REV strains in Japanese quail embryos during active infection, whereas these assays were much less sensitive when applied to archived tissue samples from chronically infected birds with lymphoid tumours. The diagnostic assays developed in this study have potential as diagnostic tools for detection of active REV infections.

Original languageEnglish (US)
Pages (from-to)451-456
Number of pages6
JournalAvian Pathology
Volume37
Issue number4
DOIs
StatePublished - Aug 2008

Fingerprint

Reticuloendotheliosis virus
Coturnix
formalin
Paraffin
alkanes
in situ hybridization
Formaldehyde
In Situ Hybridization
immunohistochemistry
embryo (animal)
Embryonic Structures
Immunohistochemistry
Virus Diseases
neoplasms
Neoplasms
infection
assays
Birds
Avian Leukosis
reticuloendotheliosis

ASJC Scopus subject areas

  • Immunology
  • veterinary(all)

Cite this

Detection of reticuloendotheliosis virus by immunohistochemistry and in situ hybridization in experimentally infected Japanese quail embryos and archived formalin-fixed and paraffin-embedded tumours. / Santos, Vitor L S L; Williams, Susan M.; Zavala, Guillermo; Barbosa, Taylor; Zhang, Jian; Cheng, Sunny; Shivaprasad, H L; Hafner, Scott; Fadly, Aly; Santos, Renato L.; Brown, Corrie C.

In: Avian Pathology, Vol. 37, No. 4, 08.2008, p. 451-456.

Research output: Contribution to journalArticle

Santos, Vitor L S L ; Williams, Susan M. ; Zavala, Guillermo ; Barbosa, Taylor ; Zhang, Jian ; Cheng, Sunny ; Shivaprasad, H L ; Hafner, Scott ; Fadly, Aly ; Santos, Renato L. ; Brown, Corrie C. / Detection of reticuloendotheliosis virus by immunohistochemistry and in situ hybridization in experimentally infected Japanese quail embryos and archived formalin-fixed and paraffin-embedded tumours. In: Avian Pathology. 2008 ; Vol. 37, No. 4. pp. 451-456.
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