Detection of prothymosin alpha in oocytes and embryos of Bufo arenarum

Gustavo Barisone, Felix V. Vega, Fernando Domingnez, Marcelo O. Cabada

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Prothymosin alpha (PTA) was detected by immunocytological and biochemical methods in oocytes at different stages of oogenesis, and in early embryos of the amphibian Bufo anenarum. In all cases PTA was detected in the nucleus and was absent from the cytoplasm. This indicates that this protein could act at the level of regulating transcription. Western blots were carried out using polyclonal antibodies with extracts of embryos at different stages of development from early fertilisation up to neural tube. With this method PTA was detected in all the samples under study.

Original languageEnglish (US)
Pages (from-to)347-350
Number of pages4
JournalZygote
Volume6
Issue number4
DOIs
StatePublished - Jan 1 1998
Externally publishedYes

Keywords

  • Bufo arenarum
  • Embryos
  • Nucleus
  • Oocytes
  • Prothymosin alpha

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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    Barisone, G., Vega, F. V., Domingnez, F., & Cabada, M. O. (1998). Detection of prothymosin alpha in oocytes and embryos of Bufo arenarum. Zygote, 6(4), 347-350. https://doi.org/10.1017/S0967199498000306