Detection of prothymosin alpha in oocytes and embryos of Bufo arenarum

Gustavo Barisone; Felix V. Vega; Fernando Domingnez; Marcelo O. Cabada

doi:10.1017/S0967199498000306

Detection of prothymosin alpha in oocytes and embryos of Bufo arenarum

Gustavo Barisone, Felix V. Vega, Fernando Domingnez, Marcelo O. Cabada

Research output: Contribution to journal › Article › peer-review

1 Scopus citations

Abstract

Prothymosin alpha (PTA) was detected by immunocytological and biochemical methods in oocytes at different stages of oogenesis, and in early embryos of the amphibian Bufo anenarum. In all cases PTA was detected in the nucleus and was absent from the cytoplasm. This indicates that this protein could act at the level of regulating transcription. Western blots were carried out using polyclonal antibodies with extracts of embryos at different stages of development from early fertilisation up to neural tube. With this method PTA was detected in all the samples under study.

Original language	English (US)
Pages (from-to)	347-350
Number of pages	4
Journal	Zygote
Volume	6
Issue number	4
DOIs	https://doi.org/10.1017/S0967199498000306
State	Published - Jan 1 1998
Externally published	Yes

Keywords

Bufo arenarum
Embryos
Nucleus
Oocytes
Prothymosin alpha

ASJC Scopus subject areas

Developmental Biology
Cell Biology

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title = "Detection of prothymosin alpha in oocytes and embryos of Bufo arenarum",

abstract = "Prothymosin alpha (PTA) was detected by immunocytological and biochemical methods in oocytes at different stages of oogenesis, and in early embryos of the amphibian Bufo anenarum. In all cases PTA was detected in the nucleus and was absent from the cytoplasm. This indicates that this protein could act at the level of regulating transcription. Western blots were carried out using polyclonal antibodies with extracts of embryos at different stages of development from early fertilisation up to neural tube. With this method PTA was detected in all the samples under study.",

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author = "Gustavo Barisone and Vega, {Felix V.} and Fernando Domingnez and Cabada, {Marcelo O.}",

year = "1998",

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T1 - Detection of prothymosin alpha in oocytes and embryos of Bufo arenarum

AU - Barisone, Gustavo

AU - Vega, Felix V.

AU - Domingnez, Fernando

AU - Cabada, Marcelo O.

PY - 1998/1/1

Y1 - 1998/1/1

N2 - Prothymosin alpha (PTA) was detected by immunocytological and biochemical methods in oocytes at different stages of oogenesis, and in early embryos of the amphibian Bufo anenarum. In all cases PTA was detected in the nucleus and was absent from the cytoplasm. This indicates that this protein could act at the level of regulating transcription. Western blots were carried out using polyclonal antibodies with extracts of embryos at different stages of development from early fertilisation up to neural tube. With this method PTA was detected in all the samples under study.

AB - Prothymosin alpha (PTA) was detected by immunocytological and biochemical methods in oocytes at different stages of oogenesis, and in early embryos of the amphibian Bufo anenarum. In all cases PTA was detected in the nucleus and was absent from the cytoplasm. This indicates that this protein could act at the level of regulating transcription. Western blots were carried out using polyclonal antibodies with extracts of embryos at different stages of development from early fertilisation up to neural tube. With this method PTA was detected in all the samples under study.

KW - Bufo arenarum

KW - Embryos

KW - Nucleus

KW - Oocytes

KW - Prothymosin alpha

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