Abstract
Twelve volunteer plasma donors were studied so as to determine the extent and duration of in vivo platelet activation caused by automated plasmapheresis. Samples obtained immediately before and after donation were mixed with murine monoclonal antibodies PAC-1 and S12, which bind specifically to activated platelets. Antibody binding on platelets was quantitated by flow cytometry. The change in the mean fluorescence intensity (MFI) (MFI after donation minus MFI before donation) was 61 ± 23 (confidence interval [CI], 48-75) for PAC-1 and 56 ± 64 (CI, 19-92) for S12 in plasmapheresis donors, as compared to 0.3 ± 0.8 (PAC-1: CI, -0.2-0.8) and 0.3 ± 0.9 (S12: CI, -0.3-0.9) in whole blood donors (p<0.05). Additional studies showed circulating activated platelets up to 48 hours after plasmapheresis. In contrast to other data, significant platelet activation was demonstrated following plasmapheresis on an automated machine. None of the donors had clinical complications. Nevertheless, it may be appropriate to delay subsequent plasmapheresis and platelet procurement from such donors until evidence of platelet activation has disappeared.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 534-540 |
| Number of pages | 7 |
| Journal | Transfusion |
| Volume | 32 |
| Issue number | 6 |
| State | Published - 1992 |
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ASJC Scopus subject areas
- Immunology
- Hematology
Cite this
Detection of plasmapheresis-induced platelet activation using monoclonal antibodies. / Wun, Theodore; Paglieroni, T.; Sazama, K.; Holland, P.
In: Transfusion, Vol. 32, No. 6, 1992, p. 534-540.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of plasmapheresis-induced platelet activation using monoclonal antibodies
AU - Wun, Theodore
AU - Paglieroni, T.
AU - Sazama, K.
AU - Holland, P.
PY - 1992
Y1 - 1992
N2 - Twelve volunteer plasma donors were studied so as to determine the extent and duration of in vivo platelet activation caused by automated plasmapheresis. Samples obtained immediately before and after donation were mixed with murine monoclonal antibodies PAC-1 and S12, which bind specifically to activated platelets. Antibody binding on platelets was quantitated by flow cytometry. The change in the mean fluorescence intensity (MFI) (MFI after donation minus MFI before donation) was 61 ± 23 (confidence interval [CI], 48-75) for PAC-1 and 56 ± 64 (CI, 19-92) for S12 in plasmapheresis donors, as compared to 0.3 ± 0.8 (PAC-1: CI, -0.2-0.8) and 0.3 ± 0.9 (S12: CI, -0.3-0.9) in whole blood donors (p<0.05). Additional studies showed circulating activated platelets up to 48 hours after plasmapheresis. In contrast to other data, significant platelet activation was demonstrated following plasmapheresis on an automated machine. None of the donors had clinical complications. Nevertheless, it may be appropriate to delay subsequent plasmapheresis and platelet procurement from such donors until evidence of platelet activation has disappeared.
AB - Twelve volunteer plasma donors were studied so as to determine the extent and duration of in vivo platelet activation caused by automated plasmapheresis. Samples obtained immediately before and after donation were mixed with murine monoclonal antibodies PAC-1 and S12, which bind specifically to activated platelets. Antibody binding on platelets was quantitated by flow cytometry. The change in the mean fluorescence intensity (MFI) (MFI after donation minus MFI before donation) was 61 ± 23 (confidence interval [CI], 48-75) for PAC-1 and 56 ± 64 (CI, 19-92) for S12 in plasmapheresis donors, as compared to 0.3 ± 0.8 (PAC-1: CI, -0.2-0.8) and 0.3 ± 0.9 (S12: CI, -0.3-0.9) in whole blood donors (p<0.05). Additional studies showed circulating activated platelets up to 48 hours after plasmapheresis. In contrast to other data, significant platelet activation was demonstrated following plasmapheresis on an automated machine. None of the donors had clinical complications. Nevertheless, it may be appropriate to delay subsequent plasmapheresis and platelet procurement from such donors until evidence of platelet activation has disappeared.
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M3 - Article
C2 - 1502706
AN - SCOPUS:0026730996
VL - 32
SP - 534
EP - 540
JO - Transfusion
JF - Transfusion
SN - 0041-1132
IS - 6
ER -