Detection of myoglobin desaturation in Mirounga angustirostris during apnea

Paul J. Ponganis, Ulrike Kreutzer, Napapon Sailasuta, Torre Knower, Ralph Hurd, Thomas Jue

Research output: Contribution to journalArticle

26 Scopus citations

Abstract

1H NMR solution-state study of elephant seal (Mirounga angustirostris) myoglobin (Mb) and hemoglobin (Hb) establishes the temperature-dependent chemical shifts of the proximal histidyl N δH signal, which reflects the respective intracellular and vascular Po 2 in vivo. Both proteins exist predominantly in one major isoform and do not exhibit any conformational heterogeneity. The Mb and Hb signals are detectable in M. angustirostris tissue in vivo. During eupnea M. angustirostris muscle maintains a well-saturated MbO 2. However, during apnea, the deoxymyoglobin proximal histidyl N δH signal becomes visible, reflecting a declining tissue Po 2. The study establishes a firm methodological basis for using NMR to investigate the metabolic responses during sleep apnea of the elephant seal and to secure insights into oxygen regulation in diving mammals.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Regulatory Integrative and Comparative Physiology
Volume282
Issue number1 51-1
StatePublished - 2002

Keywords

  • Apnea
  • Eupnea
  • Hypoxia
  • Nuclear magnetic resonance
  • Oxygen
  • Seal

ASJC Scopus subject areas

  • Physiology

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