Detection of Lyme disease bacterium, Borrelia burgdorferi sensu Lato, in blacklegged ticks collected in the Grand River valley, Ontario, Canada

John D. Scott, Janet E Foley, John F. Anderson, Kerry L. Clark, Lance A. Durden

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

We document the presence of blacklegged ticks, Ixodes scapularis, in the Grand River valley, Centre Wellington, Ontario. Overall, 15 (36%) of 42 I. scapularis adults collected from 41 mammalian hosts (dogs, cats, humans) were positive for the Lyme disease bacterium, Borrelia burgdorferi sensu lato (s.l.). Using real-time PCR testing and DNA sequencing of the flagellin (fla) gene, we determined that Borrelia amplicons extracted from I. scapularis adults belonged to B. burgdorferi sensu stricto (s.s.), which is pathogenic to humans and certain domestic animals. Based on the distribution of I. scapularis adults within the river basin, it appears likely that migratory birds provide an annual influx of I. scapularis immatures during northward spring migration. Health-care providers need to be aware that local residents can present with Lyme disease symptoms anytime during the year.

Original languageEnglish (US)
Pages (from-to)150-158
Number of pages9
JournalInternational Journal of Medical Sciences
Volume14
Issue number2
DOIs
StatePublished - Feb 8 2017

Fingerprint

Borrelia burgdorferi Group
Ixodes
Lyme Disease
Ontario
Ticks
Rivers
Canada
Bacteria
Borrelia
Flagellin
Borrelia burgdorferi
Domestic Animals
DNA Sequence Analysis
Health Personnel
Birds
Real-Time Polymerase Chain Reaction
Cats
Dogs
Genes

Keywords

  • Blacklegged tick
  • Borrelia burgdorferi
  • Grand River valley
  • Infection prevalence
  • Ixodes scapularis
  • Lyme disease

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Detection of Lyme disease bacterium, Borrelia burgdorferi sensu Lato, in blacklegged ticks collected in the Grand River valley, Ontario, Canada. / Scott, John D.; Foley, Janet E; Anderson, John F.; Clark, Kerry L.; Durden, Lance A.

In: International Journal of Medical Sciences, Vol. 14, No. 2, 08.02.2017, p. 150-158.

Research output: Contribution to journalArticle

@article{17794f6a1cc649f987219af89e85e63e,
title = "Detection of Lyme disease bacterium, Borrelia burgdorferi sensu Lato, in blacklegged ticks collected in the Grand River valley, Ontario, Canada",
abstract = "We document the presence of blacklegged ticks, Ixodes scapularis, in the Grand River valley, Centre Wellington, Ontario. Overall, 15 (36{\%}) of 42 I. scapularis adults collected from 41 mammalian hosts (dogs, cats, humans) were positive for the Lyme disease bacterium, Borrelia burgdorferi sensu lato (s.l.). Using real-time PCR testing and DNA sequencing of the flagellin (fla) gene, we determined that Borrelia amplicons extracted from I. scapularis adults belonged to B. burgdorferi sensu stricto (s.s.), which is pathogenic to humans and certain domestic animals. Based on the distribution of I. scapularis adults within the river basin, it appears likely that migratory birds provide an annual influx of I. scapularis immatures during northward spring migration. Health-care providers need to be aware that local residents can present with Lyme disease symptoms anytime during the year.",
keywords = "Blacklegged tick, Borrelia burgdorferi, Grand River valley, Infection prevalence, Ixodes scapularis, Lyme disease",
author = "Scott, {John D.} and Foley, {Janet E} and Anderson, {John F.} and Clark, {Kerry L.} and Durden, {Lance A.}",
year = "2017",
month = "2",
day = "8",
doi = "10.7150/ijms.17763",
language = "English (US)",
volume = "14",
pages = "150--158",
journal = "International Journal of Medical Sciences",
issn = "1449-1907",
publisher = "Ivyspring International Publisher",
number = "2",

}

TY - JOUR

T1 - Detection of Lyme disease bacterium, Borrelia burgdorferi sensu Lato, in blacklegged ticks collected in the Grand River valley, Ontario, Canada

AU - Scott, John D.

AU - Foley, Janet E

AU - Anderson, John F.

AU - Clark, Kerry L.

AU - Durden, Lance A.

PY - 2017/2/8

Y1 - 2017/2/8

N2 - We document the presence of blacklegged ticks, Ixodes scapularis, in the Grand River valley, Centre Wellington, Ontario. Overall, 15 (36%) of 42 I. scapularis adults collected from 41 mammalian hosts (dogs, cats, humans) were positive for the Lyme disease bacterium, Borrelia burgdorferi sensu lato (s.l.). Using real-time PCR testing and DNA sequencing of the flagellin (fla) gene, we determined that Borrelia amplicons extracted from I. scapularis adults belonged to B. burgdorferi sensu stricto (s.s.), which is pathogenic to humans and certain domestic animals. Based on the distribution of I. scapularis adults within the river basin, it appears likely that migratory birds provide an annual influx of I. scapularis immatures during northward spring migration. Health-care providers need to be aware that local residents can present with Lyme disease symptoms anytime during the year.

AB - We document the presence of blacklegged ticks, Ixodes scapularis, in the Grand River valley, Centre Wellington, Ontario. Overall, 15 (36%) of 42 I. scapularis adults collected from 41 mammalian hosts (dogs, cats, humans) were positive for the Lyme disease bacterium, Borrelia burgdorferi sensu lato (s.l.). Using real-time PCR testing and DNA sequencing of the flagellin (fla) gene, we determined that Borrelia amplicons extracted from I. scapularis adults belonged to B. burgdorferi sensu stricto (s.s.), which is pathogenic to humans and certain domestic animals. Based on the distribution of I. scapularis adults within the river basin, it appears likely that migratory birds provide an annual influx of I. scapularis immatures during northward spring migration. Health-care providers need to be aware that local residents can present with Lyme disease symptoms anytime during the year.

KW - Blacklegged tick

KW - Borrelia burgdorferi

KW - Grand River valley

KW - Infection prevalence

KW - Ixodes scapularis

KW - Lyme disease

UR - http://www.scopus.com/inward/record.url?scp=85011990709&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85011990709&partnerID=8YFLogxK

U2 - 10.7150/ijms.17763

DO - 10.7150/ijms.17763

M3 - Article

C2 - 28260991

AN - SCOPUS:85011990709

VL - 14

SP - 150

EP - 158

JO - International Journal of Medical Sciences

JF - International Journal of Medical Sciences

SN - 1449-1907

IS - 2

ER -