Detection of Lyme disease bacterium, Borrelia burgdorferi sensu Lato, in blacklegged ticks collected in the Grand River valley, Ontario, Canada

John D. Scott, Janet E Foley, John F. Anderson, Kerry L. Clark, Lance A. Durden

Research output: Contribution to journalArticle

4 Scopus citations


We document the presence of blacklegged ticks, Ixodes scapularis, in the Grand River valley, Centre Wellington, Ontario. Overall, 15 (36%) of 42 I. scapularis adults collected from 41 mammalian hosts (dogs, cats, humans) were positive for the Lyme disease bacterium, Borrelia burgdorferi sensu lato (s.l.). Using real-time PCR testing and DNA sequencing of the flagellin (fla) gene, we determined that Borrelia amplicons extracted from I. scapularis adults belonged to B. burgdorferi sensu stricto (s.s.), which is pathogenic to humans and certain domestic animals. Based on the distribution of I. scapularis adults within the river basin, it appears likely that migratory birds provide an annual influx of I. scapularis immatures during northward spring migration. Health-care providers need to be aware that local residents can present with Lyme disease symptoms anytime during the year.

Original languageEnglish (US)
Pages (from-to)150-158
Number of pages9
JournalInternational Journal of Medical Sciences
Issue number2
StatePublished - Feb 8 2017



  • Blacklegged tick
  • Borrelia burgdorferi
  • Grand River valley
  • Infection prevalence
  • Ixodes scapularis
  • Lyme disease

ASJC Scopus subject areas

  • Medicine(all)

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