Detection of intestinal protozoa in the clinical laboratory

Ian Howard Mchardy, Max Wu, Robyn Shimizu-Cohen, Marc Roger Couturier, Romney M. Humphries

Research output: Contribution to journalReview article

77 Citations (Scopus)

Abstract

Despite recent advances in diagnostic technology, microscopic examination of stool specimens remains central to the diagnosis of most pathogenic intestinal protozoa. Microscopy is, however, labor-intensive and requires a skilled technologist. New, highly sensitive diagnostic methods have been developed for protozoa endemic to developed countries, including Giardia lamblia (syn. G. intestinalis/G. duodenalis) and Cryptosporidium spp., using technologies that, if expanded, could effectively complement or even replace microscopic approaches. To date, the scope of such novel technologies is limited and may not include common protozoa such as Dientamoeba fragilis, Entamoeba histolytica, or Cyclospora cayetanensis. This minireview describes canonical approaches for the detection of pathogenic intestinal protozoa, while highlighting recent developments and FDA-approved tools for clinical diagnosis of common intestinal protozoa.

Original languageEnglish (US)
Pages (from-to)712-720
Number of pages9
JournalJournal of Clinical Microbiology
Volume52
Issue number3
DOIs
StatePublished - 2014

Fingerprint

Technology
Dientamoeba
Cyclospora
Giardia lamblia
Cryptosporidium
Entamoeba histolytica
Developed Countries
Microscopy

ASJC Scopus subject areas

  • Microbiology (medical)

Cite this

Mchardy, I. H., Wu, M., Shimizu-Cohen, R., Roger Couturier, M., & Humphries, R. M. (2014). Detection of intestinal protozoa in the clinical laboratory. Journal of Clinical Microbiology, 52(3), 712-720. https://doi.org/10.1128/JCM.02877-13

Detection of intestinal protozoa in the clinical laboratory. / Mchardy, Ian Howard; Wu, Max; Shimizu-Cohen, Robyn; Roger Couturier, Marc; Humphries, Romney M.

In: Journal of Clinical Microbiology, Vol. 52, No. 3, 2014, p. 712-720.

Research output: Contribution to journalReview article

Mchardy, IH, Wu, M, Shimizu-Cohen, R, Roger Couturier, M & Humphries, RM 2014, 'Detection of intestinal protozoa in the clinical laboratory', Journal of Clinical Microbiology, vol. 52, no. 3, pp. 712-720. https://doi.org/10.1128/JCM.02877-13
Mchardy, Ian Howard ; Wu, Max ; Shimizu-Cohen, Robyn ; Roger Couturier, Marc ; Humphries, Romney M. / Detection of intestinal protozoa in the clinical laboratory. In: Journal of Clinical Microbiology. 2014 ; Vol. 52, No. 3. pp. 712-720.
@article{217637cb9e4a4473b7f4afa01f17d8ab,
title = "Detection of intestinal protozoa in the clinical laboratory",
abstract = "Despite recent advances in diagnostic technology, microscopic examination of stool specimens remains central to the diagnosis of most pathogenic intestinal protozoa. Microscopy is, however, labor-intensive and requires a skilled technologist. New, highly sensitive diagnostic methods have been developed for protozoa endemic to developed countries, including Giardia lamblia (syn. G. intestinalis/G. duodenalis) and Cryptosporidium spp., using technologies that, if expanded, could effectively complement or even replace microscopic approaches. To date, the scope of such novel technologies is limited and may not include common protozoa such as Dientamoeba fragilis, Entamoeba histolytica, or Cyclospora cayetanensis. This minireview describes canonical approaches for the detection of pathogenic intestinal protozoa, while highlighting recent developments and FDA-approved tools for clinical diagnosis of common intestinal protozoa.",
author = "Mchardy, {Ian Howard} and Max Wu and Robyn Shimizu-Cohen and {Roger Couturier}, Marc and Humphries, {Romney M.}",
year = "2014",
doi = "10.1128/JCM.02877-13",
language = "English (US)",
volume = "52",
pages = "712--720",
journal = "Journal of Clinical Microbiology",
issn = "0095-1137",
publisher = "American Society for Microbiology",
number = "3",

}

TY - JOUR

T1 - Detection of intestinal protozoa in the clinical laboratory

AU - Mchardy, Ian Howard

AU - Wu, Max

AU - Shimizu-Cohen, Robyn

AU - Roger Couturier, Marc

AU - Humphries, Romney M.

PY - 2014

Y1 - 2014

N2 - Despite recent advances in diagnostic technology, microscopic examination of stool specimens remains central to the diagnosis of most pathogenic intestinal protozoa. Microscopy is, however, labor-intensive and requires a skilled technologist. New, highly sensitive diagnostic methods have been developed for protozoa endemic to developed countries, including Giardia lamblia (syn. G. intestinalis/G. duodenalis) and Cryptosporidium spp., using technologies that, if expanded, could effectively complement or even replace microscopic approaches. To date, the scope of such novel technologies is limited and may not include common protozoa such as Dientamoeba fragilis, Entamoeba histolytica, or Cyclospora cayetanensis. This minireview describes canonical approaches for the detection of pathogenic intestinal protozoa, while highlighting recent developments and FDA-approved tools for clinical diagnosis of common intestinal protozoa.

AB - Despite recent advances in diagnostic technology, microscopic examination of stool specimens remains central to the diagnosis of most pathogenic intestinal protozoa. Microscopy is, however, labor-intensive and requires a skilled technologist. New, highly sensitive diagnostic methods have been developed for protozoa endemic to developed countries, including Giardia lamblia (syn. G. intestinalis/G. duodenalis) and Cryptosporidium spp., using technologies that, if expanded, could effectively complement or even replace microscopic approaches. To date, the scope of such novel technologies is limited and may not include common protozoa such as Dientamoeba fragilis, Entamoeba histolytica, or Cyclospora cayetanensis. This minireview describes canonical approaches for the detection of pathogenic intestinal protozoa, while highlighting recent developments and FDA-approved tools for clinical diagnosis of common intestinal protozoa.

UR - http://www.scopus.com/inward/record.url?scp=84894552763&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84894552763&partnerID=8YFLogxK

U2 - 10.1128/JCM.02877-13

DO - 10.1128/JCM.02877-13

M3 - Review article

VL - 52

SP - 712

EP - 720

JO - Journal of Clinical Microbiology

JF - Journal of Clinical Microbiology

SN - 0095-1137

IS - 3

ER -