Abstract
Despite recent advances in diagnostic technology, microscopic examination of stool specimens remains central to the diagnosis of most pathogenic intestinal protozoa. Microscopy is, however, labor-intensive and requires a skilled technologist. New, highly sensitive diagnostic methods have been developed for protozoa endemic to developed countries, including Giardia lamblia (syn. G. intestinalis/G. duodenalis) and Cryptosporidium spp., using technologies that, if expanded, could effectively complement or even replace microscopic approaches. To date, the scope of such novel technologies is limited and may not include common protozoa such as Dientamoeba fragilis, Entamoeba histolytica, or Cyclospora cayetanensis. This minireview describes canonical approaches for the detection of pathogenic intestinal protozoa, while highlighting recent developments and FDA-approved tools for clinical diagnosis of common intestinal protozoa.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 712-720 |
| Number of pages | 9 |
| Journal | Journal of Clinical Microbiology |
| Volume | 52 |
| Issue number | 3 |
| DOIs | |
| State | Published - 2014 |
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ASJC Scopus subject areas
- Microbiology (medical)
Cite this
Detection of intestinal protozoa in the clinical laboratory. / Mchardy, Ian Howard; Wu, Max; Shimizu-Cohen, Robyn; Roger Couturier, Marc; Humphries, Romney M.
In: Journal of Clinical Microbiology, Vol. 52, No. 3, 2014, p. 712-720.Research output: Contribution to journal › Review article
}
TY - JOUR
T1 - Detection of intestinal protozoa in the clinical laboratory
AU - Mchardy, Ian Howard
AU - Wu, Max
AU - Shimizu-Cohen, Robyn
AU - Roger Couturier, Marc
AU - Humphries, Romney M.
PY - 2014
Y1 - 2014
N2 - Despite recent advances in diagnostic technology, microscopic examination of stool specimens remains central to the diagnosis of most pathogenic intestinal protozoa. Microscopy is, however, labor-intensive and requires a skilled technologist. New, highly sensitive diagnostic methods have been developed for protozoa endemic to developed countries, including Giardia lamblia (syn. G. intestinalis/G. duodenalis) and Cryptosporidium spp., using technologies that, if expanded, could effectively complement or even replace microscopic approaches. To date, the scope of such novel technologies is limited and may not include common protozoa such as Dientamoeba fragilis, Entamoeba histolytica, or Cyclospora cayetanensis. This minireview describes canonical approaches for the detection of pathogenic intestinal protozoa, while highlighting recent developments and FDA-approved tools for clinical diagnosis of common intestinal protozoa.
AB - Despite recent advances in diagnostic technology, microscopic examination of stool specimens remains central to the diagnosis of most pathogenic intestinal protozoa. Microscopy is, however, labor-intensive and requires a skilled technologist. New, highly sensitive diagnostic methods have been developed for protozoa endemic to developed countries, including Giardia lamblia (syn. G. intestinalis/G. duodenalis) and Cryptosporidium spp., using technologies that, if expanded, could effectively complement or even replace microscopic approaches. To date, the scope of such novel technologies is limited and may not include common protozoa such as Dientamoeba fragilis, Entamoeba histolytica, or Cyclospora cayetanensis. This minireview describes canonical approaches for the detection of pathogenic intestinal protozoa, while highlighting recent developments and FDA-approved tools for clinical diagnosis of common intestinal protozoa.
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UR - http://www.scopus.com/inward/citedby.url?scp=84894552763&partnerID=8YFLogxK
U2 - 10.1128/JCM.02877-13
DO - 10.1128/JCM.02877-13
M3 - Review article
VL - 52
SP - 712
EP - 720
JO - Journal of Clinical Microbiology
JF - Journal of Clinical Microbiology
SN - 0095-1137
IS - 3
ER -