Detection of HIV-1-specific gastrointestinal tissue resident CD8 + T-cells in chronic infection

Brenna E. Kiniry, Shengbin Li, Anupama Ganesh, Peter W. Hunt, Ma Somsouk, Pamela J. Skinner, Steven G. Deeks, Barbara Shacklett

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

Tissue-resident memory (T RM) CD8 + T-cells are non-recirculating, long-lived cells housed in tissues that can confer protection against mucosal pathogens. Human immunodeficiency virus-1 (HIV-1) is a mucosal pathogen and the gastrointestinal tract is an important site of viral pathogenesis and transmission. Thus, CD8 + T RM cells may be an important effector subset for controlling HIV-1 in mucosal tissues. This study sought to determine the abundance, phenotype, and functionality of CD8 + T RM cells in the context of chronic HIV-1 infection. We found that the majority of rectosigmoid CD8 + T-cells were CD69 + CD103 + S1PR1 ' and T-bet Low Eomesodermin Neg, indicative of a tissue-residency phenotype similar to that described in murine models. HIV-1-specific CD8 + T RM responses appeared strongest in individuals naturally controlling HIV-1 infection. Two CD8 + T RM subsets, distinguished by CD103 expression intensity, were identified. CD103 Low CD8 + T RM primarily displayed a transitional memory phenotype and contained HIV-1-specific cells and cells expressing high levels of Eomesodermin, whereas CD103 High CD8 + T RM primarily displayed an effector memory phenotype and were Eomesodermin Neg. These findings suggest a large fraction of CD8 + T-cells housed in the human rectosigmoid mucosa are tissue-resident and that T RM contribute to the anti-HIV-1 immune response. Further exploration of CD8 + T RM will inform development of anti-HIV-1 immune-based therapies and vaccines targeted to the mucosa.

Original languageEnglish (US)
Pages (from-to)909-920
Number of pages12
JournalMucosal Immunology
Volume11
Issue number3
DOIs
StatePublished - May 1 2018

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Fingerprint Dive into the research topics of 'Detection of HIV-1-specific gastrointestinal tissue resident CD8 + T-cells in chronic infection'. Together they form a unique fingerprint.

Cite this