Abstract
Indirect immunofluorescence was used to detect the presence of a male-specific protein (H-Y antigen) on pre-implantation ovine embryos. Eight-cell to blastocyst stage embryos were surgically collected from superovulated ewes, classified as fluorescent (H-Y positive) or non-fluorescent (H-Y negative) and either transferred to recipient ewes or karyotyped to confirm embryonic sex. H-Y antigen was detected on eight-cell through blastocyst stage embryos. Overall, 88% ( 50 57) of the embryos (eight-cell to early blastocyst stage) classified as H-Y positive or H-Y negative were male and female, respectively. Survival after transfer of embryos subjected to the H-Y antigen assay was high (63%), which supports the use of this procedure in conjunction with embryo transfer in sheep to produce pregnancies in which sex of the fetus is known.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 27-32 |
| Number of pages | 6 |
| Journal | Journal of Reproductive Immunology |
| Volume | 10 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1987 |
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Keywords
- H-Y antigen
- immunofluorescence
- sex ration
ASJC Scopus subject areas
- Immunology
- Reproductive Medicine
Cite this
Detection of histocompatibility-Y antigen : identification of sex of pre-implantation ovine embryos. / White, K. L.; Anderson, G. B.; Pashen, R. L.; Bondurant, Robert.
In: Journal of Reproductive Immunology, Vol. 10, No. 1, 01.01.1987, p. 27-32.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of histocompatibility-Y antigen
T2 - identification of sex of pre-implantation ovine embryos
AU - White, K. L.
AU - Anderson, G. B.
AU - Pashen, R. L.
AU - Bondurant, Robert
PY - 1987/1/1
Y1 - 1987/1/1
N2 - Indirect immunofluorescence was used to detect the presence of a male-specific protein (H-Y antigen) on pre-implantation ovine embryos. Eight-cell to blastocyst stage embryos were surgically collected from superovulated ewes, classified as fluorescent (H-Y positive) or non-fluorescent (H-Y negative) and either transferred to recipient ewes or karyotyped to confirm embryonic sex. H-Y antigen was detected on eight-cell through blastocyst stage embryos. Overall, 88% ( 50 57) of the embryos (eight-cell to early blastocyst stage) classified as H-Y positive or H-Y negative were male and female, respectively. Survival after transfer of embryos subjected to the H-Y antigen assay was high (63%), which supports the use of this procedure in conjunction with embryo transfer in sheep to produce pregnancies in which sex of the fetus is known.
AB - Indirect immunofluorescence was used to detect the presence of a male-specific protein (H-Y antigen) on pre-implantation ovine embryos. Eight-cell to blastocyst stage embryos were surgically collected from superovulated ewes, classified as fluorescent (H-Y positive) or non-fluorescent (H-Y negative) and either transferred to recipient ewes or karyotyped to confirm embryonic sex. H-Y antigen was detected on eight-cell through blastocyst stage embryos. Overall, 88% ( 50 57) of the embryos (eight-cell to early blastocyst stage) classified as H-Y positive or H-Y negative were male and female, respectively. Survival after transfer of embryos subjected to the H-Y antigen assay was high (63%), which supports the use of this procedure in conjunction with embryo transfer in sheep to produce pregnancies in which sex of the fetus is known.
KW - H-Y antigen
KW - immunofluorescence
KW - sex ration
UR - http://www.scopus.com/inward/record.url?scp=0023105574&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023105574&partnerID=8YFLogxK
U2 - 10.1016/0165-0378(87)90047-7
DO - 10.1016/0165-0378(87)90047-7
M3 - Article
C2 - 3295231
AN - SCOPUS:0023105574
VL - 10
SP - 27
EP - 32
JO - Journal of Reproductive Immunology
JF - Journal of Reproductive Immunology
SN - 0165-0378
IS - 1
ER -