Detection of histocompatibility-Y antigen: identification of sex of pre-implantation ovine embryos

K. L. White, G. B. Anderson, R. L. Pashen, Robert Bondurant

Research output: Contribution to journalArticle

12 Scopus citations

Abstract

Indirect immunofluorescence was used to detect the presence of a male-specific protein (H-Y antigen) on pre-implantation ovine embryos. Eight-cell to blastocyst stage embryos were surgically collected from superovulated ewes, classified as fluorescent (H-Y positive) or non-fluorescent (H-Y negative) and either transferred to recipient ewes or karyotyped to confirm embryonic sex. H-Y antigen was detected on eight-cell through blastocyst stage embryos. Overall, 88% ( 50 57) of the embryos (eight-cell to early blastocyst stage) classified as H-Y positive or H-Y negative were male and female, respectively. Survival after transfer of embryos subjected to the H-Y antigen assay was high (63%), which supports the use of this procedure in conjunction with embryo transfer in sheep to produce pregnancies in which sex of the fetus is known.

Original languageEnglish (US)
Pages (from-to)27-32
Number of pages6
JournalJournal of Reproductive Immunology
Volume10
Issue number1
DOIs
StatePublished - Jan 1 1987

Keywords

  • H-Y antigen
  • immunofluorescence
  • sex ration

ASJC Scopus subject areas

  • Immunology
  • Reproductive Medicine

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