Abstract
Indirect immunofluorescence was used to detect the presence of a male-specific protein (H-Y antigen) on pre-implantation ovine embryos. Eight-cell to blastocyst stage embryos were surgically collected from superovulated ewes, classified as fluorescent (H-Y positive) or non-fluorescent (H-Y negative) and either transferred to recipient ewes or karyotyped to confirm embryonic sex. H-Y antigen was detected on eight-cell through blastocyst stage embryos. Overall, 88% ( 50 57) of the embryos (eight-cell to early blastocyst stage) classified as H-Y positive or H-Y negative were male and female, respectively. Survival after transfer of embryos subjected to the H-Y antigen assay was high (63%), which supports the use of this procedure in conjunction with embryo transfer in sheep to produce pregnancies in which sex of the fetus is known.
Original language | English (US) |
---|---|
Pages (from-to) | 27-32 |
Number of pages | 6 |
Journal | Journal of Reproductive Immunology |
Volume | 10 |
Issue number | 1 |
DOIs | |
State | Published - Jan 1 1987 |
Keywords
- H-Y antigen
- immunofluorescence
- sex ration
ASJC Scopus subject areas
- Immunology
- Reproductive Medicine