Detection of glycosaminoglycan loss in articular cartilage by fluorescence lifetime imaging

Xiangnan Zhou, Anne K. Haudenschild, Benjamin E. Sherlock, Jerry C. Hu, Jonathan K Leach, Kyriacos A. Athanasiou, Laura Marcu

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Glycosaminoglycan (GAG) loss is an early marker of osteoarthritis, which is a clinical late stage disease that affects millions of people worldwide. The goal of our study was to evaluate the ability of a fiber-based fluorescence lifetime imaging (FLIm) technique to detect GAG loss in articular cartilage. Native bovine cartilage explants (n = 20) were exposed to 0 (control), 0.5 (low), or 1 U / mL (high) concentrations of chondroitinase ABC (cABC) to create samples with different levels of GAG loss. FLIm assessment (excitation: 355 nm; detection: channel 1: 375 to 410 nm, channel 2: 450 to 485 nm, channel 3: 530 to 565 nm) was conducted on depth-resolved cross-sections of the cartilage sample. FLIm images, validated with histology, revealed that loss of GAG resulted in a decrease of fluorescence lifetime values in channel 2 (Δ = 0.44 ns, p < 0.05) and channel 3 (Δ = 0.75 ns, p < 0.01) compared to control samples (channel 2: 6.34 ns; channel 3: 5.22 ns). Fluorescence intensity ratio values were lower in channel 1 (37%, p < 0.0001) and channel 2 (31% decrease, p < 0.0001) and higher in channel 3 (23%, p < 0.0001) relative to control samples. These results show that FLIm can detect the loss of GAG in articular cartilage and support further investigation into the feasibility of in vivo FLIm arthroscopy.

Original languageEnglish (US)
Article number126002
JournalJournal of Biomedical Optics
Volume23
Issue number12
DOIs
StatePublished - Dec 1 2018

Fingerprint

cartilage
Cartilage
Glycosaminoglycans
Fluorescence
Imaging techniques
life (durability)
fluorescence
Chondroitin ABC Lyase
Histology
histology
imaging techniques
markers
Fibers

Keywords

  • autofluorescence
  • cartilage
  • fluorescence lifetime spectroscopy and imaging
  • glycosaminoglycan
  • osteoarthritis

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Biomedical Engineering

Cite this

Zhou, X., Haudenschild, A. K., Sherlock, B. E., Hu, J. C., Leach, J. K., Athanasiou, K. A., & Marcu, L. (2018). Detection of glycosaminoglycan loss in articular cartilage by fluorescence lifetime imaging. Journal of Biomedical Optics, 23(12), [126002]. https://doi.org/10.1117/1.JBO.23.12.126002

Detection of glycosaminoglycan loss in articular cartilage by fluorescence lifetime imaging. / Zhou, Xiangnan; Haudenschild, Anne K.; Sherlock, Benjamin E.; Hu, Jerry C.; Leach, Jonathan K; Athanasiou, Kyriacos A.; Marcu, Laura.

In: Journal of Biomedical Optics, Vol. 23, No. 12, 126002, 01.12.2018.

Research output: Contribution to journalArticle

Zhou, X, Haudenschild, AK, Sherlock, BE, Hu, JC, Leach, JK, Athanasiou, KA & Marcu, L 2018, 'Detection of glycosaminoglycan loss in articular cartilage by fluorescence lifetime imaging', Journal of Biomedical Optics, vol. 23, no. 12, 126002. https://doi.org/10.1117/1.JBO.23.12.126002
Zhou, Xiangnan ; Haudenschild, Anne K. ; Sherlock, Benjamin E. ; Hu, Jerry C. ; Leach, Jonathan K ; Athanasiou, Kyriacos A. ; Marcu, Laura. / Detection of glycosaminoglycan loss in articular cartilage by fluorescence lifetime imaging. In: Journal of Biomedical Optics. 2018 ; Vol. 23, No. 12.
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