Detection of glycine receptor/Cl- channel beta subunit transcripts in mouse testis

Yoko Sato, Richard P Tucker, Stanley Meizel

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

The sperm glycine receptor/Cl- channel (GlyR) is important to the initiation of the mammalian sperm acrosome reaction by the egg zona pellucida, but its presence in spermatogenic cells has not been demonstrated. Reverse transcriptase-polymerase chain reaction studies confirmed that GlyR beta subunit transcripts similar to those of the neuronal GlyR beta subunit are present in the testis of Swiss Webster mice. In situ hybridisation analysis demonstrated that GlyR beta subunit mRNAs were expressed within the germ cells of seminiferous tubules in those mice.

Original languageEnglish (US)
Pages (from-to)105-108
Number of pages4
JournalZygote
Volume10
Issue number2
DOIs
StatePublished - May 2002

Keywords

  • Acrosome reaction
  • In situ hybridisation
  • RT-PCR
  • Sperm

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology

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