Detection of galectin-3 in tear fluid at disease states and immunohistochemical and lectin histochemical analysis in human corneal and conjunctival epithelium

E. Hrdličková-Cela, J. Plzák, Jr Smetana, Z. Mělková, H. Kaltner, M. Filipec, Fu-Tong Liu, H. J. Gabius

Research output: Contribution to journalArticle

45 Scopus citations

Abstract

Background/aim - Components of the tear fluid contribute to the biochemical defence system of the eye. To reveal whether the immune mediator and lipopolysaccharide binding galectin-3 is present in tears, tear samples were collected from eyes in healthy and pathological states. Investigation of expression of galectin-3 and galectin-3 reactive glyco-ligands in normal human conjunctival and corneal epithelia was also initiated as a step to understand the role of galectin-3 in ocular surface pathology. Methods - Immunoblot analysis using either a rabbit polyclonal or a mouse monoclonal antibody against galectin-3 was employed to detect galectin-3 in tear fluid. Galectin-3 expression in tissue specimens was detected by immunocytochemistry employing A1D6 mouse monoclonal anti-body, and galectin-3 reactive glycoligands were visualised by lectin histochemistry using labelled galectin-3. Results - Galectin-3 was found only in tears from patients with ocular surface disorders. It was expressed in normal corneal and conjunctival epithelia but not in lacrimal glands. Inflammatory leucocytes and goblet cells found in galectin-3 containing tear fluid also expressed galectin-3. Galectin-3 binding sites were detected on the surface of conjunctival and corneal epithelial cells co-localising with desmoglein. Conclusions - This study revealed expression of galectin-3 in tear fluid obtained from patients with eye diseases. The role of this endogenous lectin (produced by inflammatory as well as epithelial cells) in antimicrobial action and inflammation modulation could be expected.

Original languageEnglish (US)
Pages (from-to)1336-1340
Number of pages5
JournalBritish Journal of Ophthalmology
Volume85
Issue number11
DOIs
StatePublished - 2001

ASJC Scopus subject areas

  • Ophthalmology

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