Detection of Flexibacter maritimus by Fluorescent Antibody Technique in Experimentally Infected Black Sea Bream Fry

Dolores Baxa, Kenji Kawai, Riichi Kusuda

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Direct fluorescent antibody technique (FAT) was used in the detection of Flexibacter maritimus in experimentally infected black sea bream fry, Acanthopagrus schlegeli, by smear plus immersion. The sensitivity of the technique was compared with the conventional media culture method. Tissue sections of the skin, gill, liver and kidney were stained with fluorescent antibody. By culture method, tissues were separately homogenized, serially diluted and plate counted using Cytophaga agar in 100% seawater. Fluorescent antibody technique detected F. maritimus in all tissues from 15 min until all experimental fish died after 48 h. Whereas by culture method, the bacteria was detected in the skin only from 15 min to 3 h, in the gills from 1 h to 12 h, and in the internal organs from 15 min to 12 h but could not be detected thereafter because of the proliferation of other bacteria. Thus, FAT proved to be a more useful technique than the plate culture method in the diagnosis of induced Flexibacter infection in black sea bream fry.

Original languageEnglish (US)
Pages (from-to)29-32
Number of pages4
JournalFish Pathology
Volume23
Issue number1
DOIs
StatePublished - Jan 1 1988
Externally publishedYes

ASJC Scopus subject areas

  • Aquatic Science
  • Animal Science and Zoology

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