Detection of estrogen receptor messenger ribonucleic acid in human oocytes and cumulus-oocyte complexes using reverse transcriptase-polymerase chain reaction

J. T C Wu, L. Wang, Yu-Jui Yvonne Wan

Research output: Contribution to journalArticle

100 Citations (Scopus)

Abstract

Objectives: To examine the expression of estrogen receptor (ER) messenger ribonucleic acid in the human ovary. To detect ER gene transcripts in human oocytes; and to study the possible autocrine effect of estrogen (E) in granulosa/cumulus cells. Design: Human ovaries from benign gynecological surgeries as well as the oocytes, cumulus-oocyte complexes, and granulosa/cumulus cells from the in vitro fertilization program were used to detect ER gene transcripts using the highly sensitive reverse transcriptase- polymerase chain reaction technique. Setting: Molecular biology laboratory. Results: The ER gene is expressed in human ovary. Estrogen receptor transcripts were detected in human cumulus-oocyte complexes and oocytes but not in granulosa/cumulus cells. Conclusions: These results suggest a lack of receptor-mediated autocrine effect of E during folliculogenesis and that E, secreted by granulosa/cumulus cells, may exert a paracrine effect to influence oocyte maturation and fertilization competence directly.

Original languageEnglish (US)
Pages (from-to)54-59
Number of pages6
JournalFertility and Sterility
Volume59
Issue number1
StatePublished - 1993

Fingerprint

Reverse Transcriptase Polymerase Chain Reaction
Estrogen Receptors
Cumulus Cells
Oocytes
RNA
Ovary
Genes
Gynecologic Surgical Procedures
Fertilization in Vitro
Fertilization
Mental Competency
Molecular Biology
Estrogens

Keywords

  • cumulus
  • Estrogen receptor
  • gene expression
  • human
  • messenger ribonucleic acid
  • oocyte
  • ovary

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

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title = "Detection of estrogen receptor messenger ribonucleic acid in human oocytes and cumulus-oocyte complexes using reverse transcriptase-polymerase chain reaction",
abstract = "Objectives: To examine the expression of estrogen receptor (ER) messenger ribonucleic acid in the human ovary. To detect ER gene transcripts in human oocytes; and to study the possible autocrine effect of estrogen (E) in granulosa/cumulus cells. Design: Human ovaries from benign gynecological surgeries as well as the oocytes, cumulus-oocyte complexes, and granulosa/cumulus cells from the in vitro fertilization program were used to detect ER gene transcripts using the highly sensitive reverse transcriptase- polymerase chain reaction technique. Setting: Molecular biology laboratory. Results: The ER gene is expressed in human ovary. Estrogen receptor transcripts were detected in human cumulus-oocyte complexes and oocytes but not in granulosa/cumulus cells. Conclusions: These results suggest a lack of receptor-mediated autocrine effect of E during folliculogenesis and that E, secreted by granulosa/cumulus cells, may exert a paracrine effect to influence oocyte maturation and fertilization competence directly.",
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T1 - Detection of estrogen receptor messenger ribonucleic acid in human oocytes and cumulus-oocyte complexes using reverse transcriptase-polymerase chain reaction

AU - Wu, J. T C

AU - Wang, L.

AU - Wan, Yu-Jui Yvonne

PY - 1993

Y1 - 1993

N2 - Objectives: To examine the expression of estrogen receptor (ER) messenger ribonucleic acid in the human ovary. To detect ER gene transcripts in human oocytes; and to study the possible autocrine effect of estrogen (E) in granulosa/cumulus cells. Design: Human ovaries from benign gynecological surgeries as well as the oocytes, cumulus-oocyte complexes, and granulosa/cumulus cells from the in vitro fertilization program were used to detect ER gene transcripts using the highly sensitive reverse transcriptase- polymerase chain reaction technique. Setting: Molecular biology laboratory. Results: The ER gene is expressed in human ovary. Estrogen receptor transcripts were detected in human cumulus-oocyte complexes and oocytes but not in granulosa/cumulus cells. Conclusions: These results suggest a lack of receptor-mediated autocrine effect of E during folliculogenesis and that E, secreted by granulosa/cumulus cells, may exert a paracrine effect to influence oocyte maturation and fertilization competence directly.

AB - Objectives: To examine the expression of estrogen receptor (ER) messenger ribonucleic acid in the human ovary. To detect ER gene transcripts in human oocytes; and to study the possible autocrine effect of estrogen (E) in granulosa/cumulus cells. Design: Human ovaries from benign gynecological surgeries as well as the oocytes, cumulus-oocyte complexes, and granulosa/cumulus cells from the in vitro fertilization program were used to detect ER gene transcripts using the highly sensitive reverse transcriptase- polymerase chain reaction technique. Setting: Molecular biology laboratory. Results: The ER gene is expressed in human ovary. Estrogen receptor transcripts were detected in human cumulus-oocyte complexes and oocytes but not in granulosa/cumulus cells. Conclusions: These results suggest a lack of receptor-mediated autocrine effect of E during folliculogenesis and that E, secreted by granulosa/cumulus cells, may exert a paracrine effect to influence oocyte maturation and fertilization competence directly.

KW - cumulus

KW - Estrogen receptor

KW - gene expression

KW - human

KW - messenger ribonucleic acid

KW - oocyte

KW - ovary

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