Detection of enterotropic mouse hepatitis virus fecal excretion by polymerase chain reaction

D. B. Casebolt, B. Qian, C. B. Stephensen

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A reverse transcription and polymerase chain reaction method was developed and used to detect fecal excretion of enterotropic mouse hepatitis virus (MHV). A nested set of primers for polymerase chain reaction was located in the polymerase region of the MHV genome. The assay was used to follow the time course of enterotropic MHV excretion in mice after oronasal inoculation with the virus. The assay detected MHV genome excretion in all mice beginning on day 3 through day 21 after inoculation. No MHV genome was detected in inoculated mice on or after day 27 following inoculation. This time course of detection of excreted virus corresponded to the period of infectivity of this isolate in a previous study. These results indicate that reverse transcription and polymerase chain reaction are useful for enterotropic MHV detection in specimens that are easily and noninvasively obtained and are clinically relevant.

Original languageEnglish (US)
Pages (from-to)6-10
Number of pages5
JournalLaboratory Animal Science
Volume47
Issue number1
StatePublished - 1997
Externally publishedYes

Fingerprint

Murine hepatitis virus
Polymerase chain reaction
Viruses
excretion
polymerase chain reaction
Polymerase Chain Reaction
reverse transcription
Genome
Reverse Transcription
genome
Genes
mice
Transcription
Assays
viruses
assays
pathogenicity

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Detection of enterotropic mouse hepatitis virus fecal excretion by polymerase chain reaction. / Casebolt, D. B.; Qian, B.; Stephensen, C. B.

In: Laboratory Animal Science, Vol. 47, No. 1, 1997, p. 6-10.

Research output: Contribution to journalArticle

Casebolt, D. B. ; Qian, B. ; Stephensen, C. B. / Detection of enterotropic mouse hepatitis virus fecal excretion by polymerase chain reaction. In: Laboratory Animal Science. 1997 ; Vol. 47, No. 1. pp. 6-10.
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