Detection of elevated βhydroxybutyrate in the plasma of fasted and alloxan-treated hamsters by h NMR

Mannfred A. Hollinger, Shri Giri, Ryan Mitchell, Thomas Jue

Research output: Contribution to journalArticle

Abstract

The application of nuclear magnetic resonance (NMR) spectroscopy to the analysis of tissue samples is often complicated by the presence of interfering signals. A plasma metabolite of considerable biological significance is βhydroxy-butyrate (βHB), which can reflect altered lipid and carbohydrate metabolism. Direct measurement of βHB in plasma via NMR is nullified by the presence of predominating lipid signals. The present study reports the successful application of a spectral editing sequence to measure elevated plasma βHB levels in fasted and alloxan-treated hamsters.

Original languageEnglish (US)
Pages (from-to)102-108
Number of pages7
JournalToxicology Mechanisms and Methods
Volume4
Issue number2
DOIs
StatePublished - 1994

Keywords

  • Alloxan
  • Fasting
  • Nuclear magnetic resonance
  • βHydroxybutyrate

ASJC Scopus subject areas

  • Toxicology
  • Health, Toxicology and Mutagenesis

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