Detection of Ehrlichia risticii, the agent of potomac horse fever, in freshwater stream snails (Pleuroceridae: Juga spp.) from Northern California

Jeffrey E. Barlough, Gerhard H. Reubel, John E Madigan, Larisa K. Vredevoe, Paul E. Miller, Yasuko Rikihisa

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Ehrlichia DNA was identified by nested PCR in operculate snails (Pleuroceridae: Juga spp.) collected from stream water in a northern California pasture in which Potomac horse fever (PHF) is enzootic. Sequencing of PCR-amplified DNA from a suite of genes (the 16S rRNA, groESL heat shock operon, 51-kDa major antigen genes) indicated that the source organism closely resembled Ehrlichia risticii, the causative agent of PHF. The minimum percentage of Juga spp. harboring the organism in the population studied was 3.5% (2 of 57 snails). No ehrlichia DNA was found in tissues of 123 lymnaeid, physid, and planorbid snails collected at the same site. These data suggest that pleurocerid stream snails may play a role in the life cycle of E. risticii in northern California.

Original languageEnglish (US)
Pages (from-to)2888-2893
Number of pages6
JournalApplied and Environmental Microbiology
Volume64
Issue number8
StatePublished - Aug 1998

Fingerprint

Neorickettsia risticii
Snails
horse
Fresh Water
snail
Horses
snails
Fever
Ehrlichia
DNA
Potomac horse fever
gene
heat shock
antigen
Polymerase Chain Reaction
organisms
operon
pasture
Operon
Life Cycle Stages

ASJC Scopus subject areas

  • Environmental Science(all)
  • Biotechnology
  • Microbiology

Cite this

Detection of Ehrlichia risticii, the agent of potomac horse fever, in freshwater stream snails (Pleuroceridae : Juga spp.) from Northern California. / Barlough, Jeffrey E.; Reubel, Gerhard H.; Madigan, John E; Vredevoe, Larisa K.; Miller, Paul E.; Rikihisa, Yasuko.

In: Applied and Environmental Microbiology, Vol. 64, No. 8, 08.1998, p. 2888-2893.

Research output: Contribution to journalArticle

Barlough, Jeffrey E. ; Reubel, Gerhard H. ; Madigan, John E ; Vredevoe, Larisa K. ; Miller, Paul E. ; Rikihisa, Yasuko. / Detection of Ehrlichia risticii, the agent of potomac horse fever, in freshwater stream snails (Pleuroceridae : Juga spp.) from Northern California. In: Applied and Environmental Microbiology. 1998 ; Vol. 64, No. 8. pp. 2888-2893.
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