Detection of Clostridium perfringens type D epsilon antitoxin in serum of goats by competitive and indirect ELISA

Francisco A Uzal; K. Nielsen; W. R. Kelly

doi:10.1016/S0378-1135(97)00122-3

Detection of Clostridium perfringens type D epsilon antitoxin in serum of goats by competitive and indirect ELISA

Francisco A Uzal, K. Nielsen, W. R. Kelly

Research output: Contribution to journal › Article › peer-review

30 Scopus citations

Abstract

Indirect and competitive ELISA techniques were developed and their ability to detect antibodies to Clostridium perfringens epsilon toxin in goat serum was compared, Different dilutions of a hyperimmune goat serum, in serum from a colostrum-deprived kid, were used as positive controls, while sera from eleven colostrum-deprived kids were used as negative controls. The epsilon toxin antibodies in the hyperimmune serum were also measured by mouse neutralisation test (MNT). The correlation coefficient between both the indirect ELISA technique and MNT was 0.99, while the same coefficient for the competitive ELISA was 0.98. Both the indirect and competitive ELISAs proved to be rapid, simple, sensitive and specific for detecting antibodies to C. perfringens epsilon toxin in serum of goats.

Original language	English (US)
Pages (from-to)	223-231
Number of pages	9
Journal	Veterinary Microbiology
Volume	57
Issue number	2-3
DOIs	https://doi.org/10.1016/S0378-1135(97)00122-3
State	Published - Sep 1997
Externally published	Yes

Keywords

Clostridium perfringens
Diagnosis-bacteria
ELISA
Goat
Toxins

ASJC Scopus subject areas

Animal Science and Zoology
Microbiology
veterinary(all)

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title = "Detection of Clostridium perfringens type D epsilon antitoxin in serum of goats by competitive and indirect ELISA",

abstract = "Indirect and competitive ELISA techniques were developed and their ability to detect antibodies to Clostridium perfringens epsilon toxin in goat serum was compared, Different dilutions of a hyperimmune goat serum, in serum from a colostrum-deprived kid, were used as positive controls, while sera from eleven colostrum-deprived kids were used as negative controls. The epsilon toxin antibodies in the hyperimmune serum were also measured by mouse neutralisation test (MNT). The correlation coefficient between both the indirect ELISA technique and MNT was 0.99, while the same coefficient for the competitive ELISA was 0.98. Both the indirect and competitive ELISAs proved to be rapid, simple, sensitive and specific for detecting antibodies to C. perfringens epsilon toxin in serum of goats.",

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AU - Uzal, Francisco A

AU - Nielsen, K.

AU - Kelly, W. R.

PY - 1997/9

Y1 - 1997/9

N2 - Indirect and competitive ELISA techniques were developed and their ability to detect antibodies to Clostridium perfringens epsilon toxin in goat serum was compared, Different dilutions of a hyperimmune goat serum, in serum from a colostrum-deprived kid, were used as positive controls, while sera from eleven colostrum-deprived kids were used as negative controls. The epsilon toxin antibodies in the hyperimmune serum were also measured by mouse neutralisation test (MNT). The correlation coefficient between both the indirect ELISA technique and MNT was 0.99, while the same coefficient for the competitive ELISA was 0.98. Both the indirect and competitive ELISAs proved to be rapid, simple, sensitive and specific for detecting antibodies to C. perfringens epsilon toxin in serum of goats.

AB - Indirect and competitive ELISA techniques were developed and their ability to detect antibodies to Clostridium perfringens epsilon toxin in goat serum was compared, Different dilutions of a hyperimmune goat serum, in serum from a colostrum-deprived kid, were used as positive controls, while sera from eleven colostrum-deprived kids were used as negative controls. The epsilon toxin antibodies in the hyperimmune serum were also measured by mouse neutralisation test (MNT). The correlation coefficient between both the indirect ELISA technique and MNT was 0.99, while the same coefficient for the competitive ELISA was 0.98. Both the indirect and competitive ELISAs proved to be rapid, simple, sensitive and specific for detecting antibodies to C. perfringens epsilon toxin in serum of goats.

KW - Clostridium perfringens

KW - Diagnosis-bacteria

KW - ELISA

KW - Goat

KW - Toxins

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Detection of Clostridium perfringens type D epsilon antitoxin in serum of goats by competitive and indirect ELISA

Abstract

Keywords

ASJC Scopus subject areas

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