Abstract
In this study, we compared the sensitivity and specificity of three immunofluorescence techniques used to detect circulating autoantibodies in dogs with pemphigus foliaceus (PF); living keratinocyte staining on a canine keratinocyte cell line, MCA-B1, indirect immunofluorescence (IIF) on canine lip and IIF on bovine esophagus. Sera from canine PF cases were positive in four out of 27 dogs (14.8%) using living keratinocyte staining on MCA-B1 cells method, and five (18.5%) and eight sera (29.6%) using IIF on canine lip and bovine esophagus methods, respectively. By contrast, none of the 31 sera from dogs with non-pemphigus dermatoses reacted with MCA-B1 cells, whereas two (6.5%) as well as five sera (16.1%) obtained from those dogs showed positive reactivity with IIF on canine lip and bovine esophagus, respectively. Our results suggest that, although it exhibits the least sensitivity, the positive reactivity obtained by living keratinocyte staining on MCA-B1 cells can support the diagnosis of canine PF.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 105-113 |
| Number of pages | 9 |
| Journal | Research in Veterinary Science |
| Volume | 77 |
| Issue number | 2 |
| DOIs | |
| State | Published - Oct 2004 |
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Keywords
- Antibody
- Diagnosis
- Dog
- Immunofluorescence
- Keratinocyte
- Pemphigus
ASJC Scopus subject areas
- Animal Science and Zoology
- veterinary(all)
Cite this
Detection of circulating autoantibodies using living keratinocyte staining on MCA-B1 method in dogs with pemphigus foliaceus. / Honda, R.; Nishifuji, K.; Olivry, T.; White, Stephen D; Momoi, Y.; Iwasaki, T.
In: Research in Veterinary Science, Vol. 77, No. 2, 10.2004, p. 105-113.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of circulating autoantibodies using living keratinocyte staining on MCA-B1 method in dogs with pemphigus foliaceus
AU - Honda, R.
AU - Nishifuji, K.
AU - Olivry, T.
AU - White, Stephen D
AU - Momoi, Y.
AU - Iwasaki, T.
PY - 2004/10
Y1 - 2004/10
N2 - In this study, we compared the sensitivity and specificity of three immunofluorescence techniques used to detect circulating autoantibodies in dogs with pemphigus foliaceus (PF); living keratinocyte staining on a canine keratinocyte cell line, MCA-B1, indirect immunofluorescence (IIF) on canine lip and IIF on bovine esophagus. Sera from canine PF cases were positive in four out of 27 dogs (14.8%) using living keratinocyte staining on MCA-B1 cells method, and five (18.5%) and eight sera (29.6%) using IIF on canine lip and bovine esophagus methods, respectively. By contrast, none of the 31 sera from dogs with non-pemphigus dermatoses reacted with MCA-B1 cells, whereas two (6.5%) as well as five sera (16.1%) obtained from those dogs showed positive reactivity with IIF on canine lip and bovine esophagus, respectively. Our results suggest that, although it exhibits the least sensitivity, the positive reactivity obtained by living keratinocyte staining on MCA-B1 cells can support the diagnosis of canine PF.
AB - In this study, we compared the sensitivity and specificity of three immunofluorescence techniques used to detect circulating autoantibodies in dogs with pemphigus foliaceus (PF); living keratinocyte staining on a canine keratinocyte cell line, MCA-B1, indirect immunofluorescence (IIF) on canine lip and IIF on bovine esophagus. Sera from canine PF cases were positive in four out of 27 dogs (14.8%) using living keratinocyte staining on MCA-B1 cells method, and five (18.5%) and eight sera (29.6%) using IIF on canine lip and bovine esophagus methods, respectively. By contrast, none of the 31 sera from dogs with non-pemphigus dermatoses reacted with MCA-B1 cells, whereas two (6.5%) as well as five sera (16.1%) obtained from those dogs showed positive reactivity with IIF on canine lip and bovine esophagus, respectively. Our results suggest that, although it exhibits the least sensitivity, the positive reactivity obtained by living keratinocyte staining on MCA-B1 cells can support the diagnosis of canine PF.
KW - Antibody
KW - Diagnosis
KW - Dog
KW - Immunofluorescence
KW - Keratinocyte
KW - Pemphigus
UR - http://www.scopus.com/inward/record.url?scp=3242725324&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=3242725324&partnerID=8YFLogxK
U2 - 10.1016/j.rvsc.2004.03.009
DO - 10.1016/j.rvsc.2004.03.009
M3 - Article
C2 - 15196900
AN - SCOPUS:3242725324
VL - 77
SP - 105
EP - 113
JO - Research in Veterinary Science
JF - Research in Veterinary Science
SN - 0034-5288
IS - 2
ER -