Detection of circulating autoantibodies using living keratinocyte staining on MCA-B1 method in dogs with pemphigus foliaceus

R. Honda, K. Nishifuji, T. Olivry, Stephen D White, Y. Momoi, T. Iwasaki

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

In this study, we compared the sensitivity and specificity of three immunofluorescence techniques used to detect circulating autoantibodies in dogs with pemphigus foliaceus (PF); living keratinocyte staining on a canine keratinocyte cell line, MCA-B1, indirect immunofluorescence (IIF) on canine lip and IIF on bovine esophagus. Sera from canine PF cases were positive in four out of 27 dogs (14.8%) using living keratinocyte staining on MCA-B1 cells method, and five (18.5%) and eight sera (29.6%) using IIF on canine lip and bovine esophagus methods, respectively. By contrast, none of the 31 sera from dogs with non-pemphigus dermatoses reacted with MCA-B1 cells, whereas two (6.5%) as well as five sera (16.1%) obtained from those dogs showed positive reactivity with IIF on canine lip and bovine esophagus, respectively. Our results suggest that, although it exhibits the least sensitivity, the positive reactivity obtained by living keratinocyte staining on MCA-B1 cells can support the diagnosis of canine PF.

Original languageEnglish (US)
Pages (from-to)105-113
Number of pages9
JournalResearch in Veterinary Science
Volume77
Issue number2
DOIs
StatePublished - Oct 2004

Keywords

  • Antibody
  • Diagnosis
  • Dog
  • Immunofluorescence
  • Keratinocyte
  • Pemphigus

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

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