Detection of carbonic anhydrase-9 gene expression in peripheral blood cells predicts risk of disease recurrence in patients with renal cortical tumors

Scott M. Gilbert, Jared M Whitson, Mahesh Mansukhani, Ralph Buttyan, Mitchell C. Benson, Carl A. Olsson, Ihor S. Sawczuk, James M. McKiernan

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27 Scopus citations

Abstract

Objectives: To present extended follow-up on a cohort of patients with renal cortical tumors treated with partial or radical nephrectomy and preoperatively assess for carbonic anhydrase 9 tumor marker expression in the peripheral blood. Methods: All patients were originally enrolled in an institutional review board-approved study assessing the role of a reverse-transcriptase polymerase chain reaction peripheral blood assay designed to detect the tumor-specific gene carbonic anhydrase-9 (CA9). A total of 41 patients with renal cortical tumors confined to the kidney were enrolled at a single institution and assessed preoperatively with peripheral blood test for CA9 expression before undergoing partial or radical nephrectomy. A Kaplan-Meier estimated survival analysis and log-rank test were performed to determine whether detection of peripheral blood cells expressing the CA9 gene influences disease-free and disease-specific survival. Results: The median follow-up was 4.3 years. The median age was 71 years. Of the 41 patients, 26 were men and 15 were women. The estimated 5-year disease-free survival for patients with detectible expression of the CA9 gene in the peripheral blood was 39.5% compared with 88.1% for patients without detection of the CA9 gene (P = 0.048). On bivariate analysis, disease-free survival correlated with histologic type, tumor diameter, and tumor grade. Conclusions: The expression of the tumor-specific marker CA9 in the peripheral blood is associated with decreased disease-free survival in patients with renal cortical tumors. This is the first study reporting on the prognostic value of this peripheral blood-based tumor marker for kidney tumors.

Original languageEnglish (US)
Pages (from-to)942-945
Number of pages4
JournalUrology
Volume67
Issue number5
DOIs
StatePublished - May 2006
Externally publishedYes

ASJC Scopus subject areas

  • Urology

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    Gilbert, S. M., Whitson, J. M., Mansukhani, M., Buttyan, R., Benson, M. C., Olsson, C. A., Sawczuk, I. S., & McKiernan, J. M. (2006). Detection of carbonic anhydrase-9 gene expression in peripheral blood cells predicts risk of disease recurrence in patients with renal cortical tumors. Urology, 67(5), 942-945. https://doi.org/10.1016/j.urology.2005.11.034