Detection of c-sis proto-oncogene transcripts by direct enzyme-labeled cDNA probes and in situ hybridization

J. T. McClintock; I. J. Chan; S. R. Thaker; A. Katial; F. E. Taub; A. E. Aotaki-Keen; Leonard M Hjelmeland

Detection of c-sis proto-oncogene transcripts by direct enzyme-labeled cDNA probes and in situ hybridization

J. T. McClintock, I. J. Chan, S. R. Thaker, A. Katial, F. E. Taub, A. E. Aotaki-Keen, Leonard M Hjelmeland

Research output: Contribution to journal › Article › peer-review

Abstract

Using in situ hybridization and platelet-derived growth factor (PDGF) cDNA probes labeled with horseradish peroxidase, PDGF-A and -B (c-cis proto- oncogene) mRNA transcripts were identified and localized in proliferating cultures. A human retinal pigment epithelial (RPE) cell line and a glial cell line were treated with either transforming growth factor beta-1 (TGFB₁), phorbol-12-myristate-13-acetate (PMA), or thrombin from human plasma and compared for their ability to stimulate the production of PDGF-A and -B. Expression of both PDGF-A and -B transcripts were found to be localized predominantly in the cytoplasm of TGFB₁-treated RPE cells, with a portion of these cells displaying a hybridization response in the nuclear region. When compared to PMA- and thrombin-treated cells, TGFB₁ stimulated the RPE cell line to yield the greatest amount of detectable PDGF mRNA. In addition, the hybridization response observed in TGFB₁-treated cells was shown to be RNA dependent.

Original language	English (US)
Pages (from-to)	102-108
Number of pages	7
Journal	In Vitro Cellular and Developmental Biology - Animal
Volume	28 A
Issue number	2
State	Published - 1992
Externally published	Yes

ASJC Scopus subject areas

Cell Biology
Clinical Biochemistry
Developmental Biology

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Detection of c-sis proto-oncogene transcripts by direct enzyme-labeled cDNA probes and in situ hybridization. / McClintock, J. T.; Chan, I. J.; Thaker, S. R.; Katial, A.; Taub, F. E.; Aotaki-Keen, A. E.; Hjelmeland, Leonard M.

In: In Vitro Cellular and Developmental Biology - Animal, Vol. 28 A, No. 2, 1992, p. 102-108.

Research output: Contribution to journal › Article › peer-review

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title = "Detection of c-sis proto-oncogene transcripts by direct enzyme-labeled cDNA probes and in situ hybridization",

abstract = "Using in situ hybridization and platelet-derived growth factor (PDGF) cDNA probes labeled with horseradish peroxidase, PDGF-A and -B (c-cis proto- oncogene) mRNA transcripts were identified and localized in proliferating cultures. A human retinal pigment epithelial (RPE) cell line and a glial cell line were treated with either transforming growth factor beta-1 (TGFB1), phorbol-12-myristate-13-acetate (PMA), or thrombin from human plasma and compared for their ability to stimulate the production of PDGF-A and -B. Expression of both PDGF-A and -B transcripts were found to be localized predominantly in the cytoplasm of TGFB1-treated RPE cells, with a portion of these cells displaying a hybridization response in the nuclear region. When compared to PMA- and thrombin-treated cells, TGFB1 stimulated the RPE cell line to yield the greatest amount of detectable PDGF mRNA. In addition, the hybridization response observed in TGFB1-treated cells was shown to be RNA dependent.",

author = "McClintock, {J. T.} and Chan, {I. J.} and Thaker, {S. R.} and A. Katial and Taub, {F. E.} and Aotaki-Keen, {A. E.} and Hjelmeland, {Leonard M}",

year = "1992",

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AU - McClintock, J. T.

AU - Chan, I. J.

AU - Thaker, S. R.

AU - Katial, A.

AU - Taub, F. E.

AU - Aotaki-Keen, A. E.

AU - Hjelmeland, Leonard M

PY - 1992

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N2 - Using in situ hybridization and platelet-derived growth factor (PDGF) cDNA probes labeled with horseradish peroxidase, PDGF-A and -B (c-cis proto- oncogene) mRNA transcripts were identified and localized in proliferating cultures. A human retinal pigment epithelial (RPE) cell line and a glial cell line were treated with either transforming growth factor beta-1 (TGFB1), phorbol-12-myristate-13-acetate (PMA), or thrombin from human plasma and compared for their ability to stimulate the production of PDGF-A and -B. Expression of both PDGF-A and -B transcripts were found to be localized predominantly in the cytoplasm of TGFB1-treated RPE cells, with a portion of these cells displaying a hybridization response in the nuclear region. When compared to PMA- and thrombin-treated cells, TGFB1 stimulated the RPE cell line to yield the greatest amount of detectable PDGF mRNA. In addition, the hybridization response observed in TGFB1-treated cells was shown to be RNA dependent.

AB - Using in situ hybridization and platelet-derived growth factor (PDGF) cDNA probes labeled with horseradish peroxidase, PDGF-A and -B (c-cis proto- oncogene) mRNA transcripts were identified and localized in proliferating cultures. A human retinal pigment epithelial (RPE) cell line and a glial cell line were treated with either transforming growth factor beta-1 (TGFB1), phorbol-12-myristate-13-acetate (PMA), or thrombin from human plasma and compared for their ability to stimulate the production of PDGF-A and -B. Expression of both PDGF-A and -B transcripts were found to be localized predominantly in the cytoplasm of TGFB1-treated RPE cells, with a portion of these cells displaying a hybridization response in the nuclear region. When compared to PMA- and thrombin-treated cells, TGFB1 stimulated the RPE cell line to yield the greatest amount of detectable PDGF mRNA. In addition, the hybridization response observed in TGFB1-treated cells was shown to be RNA dependent.

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