Detection of c-kit mutations in canine mast cell tumors using fluorescent polyacrylamide gel electrophoresis

Cameron L R Jones, Robert A Grahn, May B. Chien, Leslie A Lyons, Cheryl A. London

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Mutations consisting of internal tandem duplications (ITDs) in exons 11 and 12 of the protooncogene c-kit are found in 30-50% of malignant canine mast cell tumors (MCTs). Traditionally, identification of such mutations in tumor specimens has been undertaken using standard polymerase chain reaction (PCR) and agarose gel electrophoresis. This procedure is limited to the detection of insertions and deletions larger than 9 base pairs in size. The purpose of this study was to compare the efficiency and accuracy of standard agarose gel electrophoresis with fluorescent polyacrylamide gel electrophoresis (PAGE) for the detection of ITDs in canine MCTs. The results of this study demonstrate that PAGE of labeled PCR products accurately predicts the size of the ITD in each tumor. In addition, other small insertions and deletions were not identified, suggesting that if they occur in canine MCTs, they do so infrequently. Because fluorescent and polyacrylamide formats are automated and have better resolution than agarose gels, fluorescent PAGE provides a more accurate, economical, and higher throughput method for the detection of c-kit mutations in canine MCTs.

Original languageEnglish (US)
Pages (from-to)95-100
Number of pages6
JournalJournal of Veterinary Diagnostic Investigation
Volume16
Issue number2
StatePublished - Mar 2004

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mast cells
Mast Cells
polyacrylamide gel electrophoresis
Canidae
Polyacrylamide Gel Electrophoresis
mutation
Mutation
neoplasms
dogs
agarose
Neoplasms
Agar Gel Electrophoresis
gel electrophoresis
polymerase chain reaction
proto-oncogenes
Polymerase Chain Reaction
polyacrylamide
Base Pairing
Sepharose
exons

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Detection of c-kit mutations in canine mast cell tumors using fluorescent polyacrylamide gel electrophoresis. / Jones, Cameron L R; Grahn, Robert A; Chien, May B.; Lyons, Leslie A; London, Cheryl A.

In: Journal of Veterinary Diagnostic Investigation, Vol. 16, No. 2, 03.2004, p. 95-100.

Research output: Contribution to journalArticle

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