Detection of bovine group B rotaviruses in feces by polymerase chain reaction

Jarasvech Chinsangaram, Geoffrey Y. Akita, Bennie Osburn

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14 Scopus citations

Abstract

A pair of primers designed from the sequence of genome segment 9 of group B rat rotavirus (IDIR) were employed to amplify genome segment 9 of a group B bovine rotavirus in a polymerase chain reaction (PCR) and to sequence the derived PCR products. A new pair of primers were synthesized from the obtained sequence data and used in a PCR detection assay for group B bovine rotavirus in fecal samples. In addition, another pair of primers were designed to produce a PCR-derived internal probe. This probe was used in a chemiluminescent hybridization to confirm the specificity and to increase the sensitivity of the assay. This assay could detect 0.1 fg of target double-stranded RNA. It was specific to group B bovine rotavirus and did not detect group B rat (IDIR) and porcine rotaviruses, group A bovine (NCDV), simian (SA-11), equine (H-2), porcine (OSU), human (DS-1), deer, and avian rotaviruses, coronavirus, or other enteric organisms tested in this study.

Original languageEnglish (US)
Pages (from-to)302-307
Number of pages6
JournalJournal of Veterinary Diagnostic Investigation
Volume6
Issue number3
DOIs
StatePublished - Jan 1 1994

ASJC Scopus subject areas

  • veterinary(all)

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