Abstract
A pair of primers designed from the sequence of genome segment 9 of group B rat rotavirus (IDIR) were employed to amplify genome segment 9 of a group B bovine rotavirus in a polymerase chain reaction (PCR) and to sequence the derived PCR products. A new pair of primers were synthesized from the obtained sequence data and used in a PCR detection assay for group B bovine rotavirus in fecal samples. In addition, another pair of primers were designed to produce a PCR-derived internal probe. This probe was used in a chemiluminescent hybridization to confirm the specificity and to increase the sensitivity of the assay. This assay could detect 0.1 fg of target double-stranded RNA. It was specific to group B bovine rotavirus and did not detect group B rat (IDIR) and porcine rotaviruses, group A bovine (NCDV), simian (SA-11), equine (H-2), porcine (OSU), human (DS-1), deer, and avian rotaviruses, coronavirus, or other enteric organisms tested in this study.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 302-307 |
| Number of pages | 6 |
| Journal | Journal of Veterinary Diagnostic Investigation |
| Volume | 6 |
| Issue number | 3 |
| DOIs | |
| State | Published - Jan 1 1994 |
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Detection of bovine group B rotaviruses in feces by polymerase chain reaction. / Chinsangaram, Jarasvech; Akita, Geoffrey Y.; Osburn, Bennie.
In: Journal of Veterinary Diagnostic Investigation, Vol. 6, No. 3, 01.01.1994, p. 302-307.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of bovine group B rotaviruses in feces by polymerase chain reaction
AU - Chinsangaram, Jarasvech
AU - Akita, Geoffrey Y.
AU - Osburn, Bennie
PY - 1994/1/1
Y1 - 1994/1/1
N2 - A pair of primers designed from the sequence of genome segment 9 of group B rat rotavirus (IDIR) were employed to amplify genome segment 9 of a group B bovine rotavirus in a polymerase chain reaction (PCR) and to sequence the derived PCR products. A new pair of primers were synthesized from the obtained sequence data and used in a PCR detection assay for group B bovine rotavirus in fecal samples. In addition, another pair of primers were designed to produce a PCR-derived internal probe. This probe was used in a chemiluminescent hybridization to confirm the specificity and to increase the sensitivity of the assay. This assay could detect 0.1 fg of target double-stranded RNA. It was specific to group B bovine rotavirus and did not detect group B rat (IDIR) and porcine rotaviruses, group A bovine (NCDV), simian (SA-11), equine (H-2), porcine (OSU), human (DS-1), deer, and avian rotaviruses, coronavirus, or other enteric organisms tested in this study.
AB - A pair of primers designed from the sequence of genome segment 9 of group B rat rotavirus (IDIR) were employed to amplify genome segment 9 of a group B bovine rotavirus in a polymerase chain reaction (PCR) and to sequence the derived PCR products. A new pair of primers were synthesized from the obtained sequence data and used in a PCR detection assay for group B bovine rotavirus in fecal samples. In addition, another pair of primers were designed to produce a PCR-derived internal probe. This probe was used in a chemiluminescent hybridization to confirm the specificity and to increase the sensitivity of the assay. This assay could detect 0.1 fg of target double-stranded RNA. It was specific to group B bovine rotavirus and did not detect group B rat (IDIR) and porcine rotaviruses, group A bovine (NCDV), simian (SA-11), equine (H-2), porcine (OSU), human (DS-1), deer, and avian rotaviruses, coronavirus, or other enteric organisms tested in this study.
UR - http://www.scopus.com/inward/record.url?scp=0028469297&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028469297&partnerID=8YFLogxK
U2 - 10.1177/104063879400600304
DO - 10.1177/104063879400600304
M3 - Article
C2 - 7948199
AN - SCOPUS:0028469297
VL - 6
SP - 302
EP - 307
JO - Journal of Veterinary Diagnostic Investigation
JF - Journal of Veterinary Diagnostic Investigation
SN - 1040-6387
IS - 3
ER -