Detection of borrelia genomospecies 2 in ixodes spinipalpis ticks collected from a rabbit in Canada

John D. Scott, Kerry L. Clark, Janet E Foley, John F. Anderson, Lance A. Durden, Jodi M. Manord, Morgan L. Smith

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Lyme disease is a serious health problem, with many patients requiring in-depth clinical assessment and extended treatment. In the present study, we provide the first records of the western blacklegged tick, Ixodes pacificus, and Ixodes spinipalpis parasitizing eastern cottontails, Sylvilagus floridanus. We also documented a triple co-infestation of 3 tick species (Ixodes angustus, I. pacificus, I. spinipalpis) feeding on an eastern cottontail. Notably, we discovered a unique member of the Lyme disease bacterium, Borrelia burgdorferi sensu lato (s.l.) in Canada. Ixodes spinipalpis ticks, which were collected from an eastern cottontail on Vancouver Island, British Columbia (BC), were positive for B. burgdorferi s.l. With the use of polymerase chain reaction amplification on the tick extracts and DNA sequencing on the borrelial amplicons, we detected Borrelia genomospecies 2, a novel subgroup of the B. burgdorferi s.l. complex. Based on 416 nucleotides of the flagellin B (flaB) gene, our amplicons are identical to the Borrelia genomospecies 2 type strain CA28. Borrelia genomospecies 2 is closely related genetically to other B. burgdorferi s.l. genospecies, namely Borrelia americana, Borrelia andersonii, and B. burgdorferi sensu stricto (s.s.) that cause Lyme disease. Like some other borrelial strains, Borrelia genomospecies 2 can be missed by current Lyme disease serology. Health-care providers must be aware that Borrelia genomospecies 2 is present in I. pacificus and I. spinipalpis ticks in far-western North America, and patients with clinical symptoms of Lyme disease need to be assessed for potential infection with this pathogen.

Original languageEnglish (US)
Pages (from-to)38-46
Number of pages9
JournalJournal of Parasitology
Volume103
Issue number1
DOIs
StatePublished - Feb 1 2017

Fingerprint

Lyme disease
Borrelia
Ixodes
tick
Ticks
Borrelia burgdorferi
Ixodes pacificus
Canada
ticks
Lyme Disease
Borrelia burgdorferi Group
rabbits
Sylvilagus
Rabbits
Borrelia andersonii
Sylvilagus floridanus
Tick Infestations
flagellin
health care
polymerase chain reaction

ASJC Scopus subject areas

  • Parasitology
  • Medicine(all)
  • Ecology, Evolution, Behavior and Systematics

Cite this

Scott, J. D., Clark, K. L., Foley, J. E., Anderson, J. F., Durden, L. A., Manord, J. M., & Smith, M. L. (2017). Detection of borrelia genomospecies 2 in ixodes spinipalpis ticks collected from a rabbit in Canada. Journal of Parasitology, 103(1), 38-46. https://doi.org/10.1645/16-127

Detection of borrelia genomospecies 2 in ixodes spinipalpis ticks collected from a rabbit in Canada. / Scott, John D.; Clark, Kerry L.; Foley, Janet E; Anderson, John F.; Durden, Lance A.; Manord, Jodi M.; Smith, Morgan L.

In: Journal of Parasitology, Vol. 103, No. 1, 01.02.2017, p. 38-46.

Research output: Contribution to journalArticle

Scott, JD, Clark, KL, Foley, JE, Anderson, JF, Durden, LA, Manord, JM & Smith, ML 2017, 'Detection of borrelia genomospecies 2 in ixodes spinipalpis ticks collected from a rabbit in Canada', Journal of Parasitology, vol. 103, no. 1, pp. 38-46. https://doi.org/10.1645/16-127
Scott, John D. ; Clark, Kerry L. ; Foley, Janet E ; Anderson, John F. ; Durden, Lance A. ; Manord, Jodi M. ; Smith, Morgan L. / Detection of borrelia genomospecies 2 in ixodes spinipalpis ticks collected from a rabbit in Canada. In: Journal of Parasitology. 2017 ; Vol. 103, No. 1. pp. 38-46.
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