Detection of bluetongue virus using a cDNA probe derived from genome segment 4 of bluetongue virus serotype 2.

J. Chinsangaram, S. Hammami, Bennie Osburn

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The double-stranded (ds) RNA genome segment 4 of bluetongue virus (BTV) serotype 2 was cloned and used as a serogroup-specific complementary (c) DNA probe for BTV diagnosis. A cDNA representing a 60% copy of genome segment 4 BTV-2 prototype was produced. The specificity of the cDNA probe was determined by hybridizing this probe to a northern blot of dsRNA (separated by polyacrylamide gel electrophoresis) of plaque-purified BTV-2 prototype. This cDNA probe was then used to hybridize to the RNA samples. Because the probe hybridized to all BTV samples but not to epizootic hemorrhagic disease virus samples, it appears to be a group-specific probe that could be used in BTV diagnosis.

Original languageEnglish (US)
Pages (from-to)8-12
Number of pages5
JournalJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Volume4
Issue number1
DOIs
StatePublished - Jan 1 1992

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Bluetongue virus
serotypes
Complementary DNA
Genome
genome
double-stranded RNA
prototypes
Epizootic hemorrhagic disease virus
Double-Stranded RNA
DNA probes
DNA Probes
sampling
Northern blotting
Northern Blotting
polyacrylamide gel electrophoresis
Serogroup
Polyacrylamide Gel Electrophoresis
RNA

ASJC Scopus subject areas

  • veterinary(all)

Cite this

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