Abstract
The avidin-biotin complex immunoperoxidase technique was adapted for use in detecting bluetongue virus (BTV) antigens in BTV serotype 11-infected bovine fetuses. Fetuses were infected with BTV serotype 11 at 120 days of gestation and then removed 20 days later by Cesarean section. Blood and tissue samples were collected from each animal and used for virus isolation in embryonated chicken eggs, the immunofluorescent antibody test, and the avidin-biotin complex test. The avidin-biotin complex method successfully identified BTV antigens in both fresh and autolyzed fetal brains. Thus, the avidin-biotin complex immunoperoxidase method has potential as a possible procedure for diagnosing bluetongue disease in aborted bovine fetuses.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 45-49 |
| Number of pages | 5 |
| Journal | Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc |
| Volume | 1 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jan 1 1989 |
| Externally published | Yes |
Fingerprint
ASJC Scopus subject areas
- veterinary(all)
Cite this
Detection of bluetongue virus in bovine fetuses using the avidin-biotin complex immunoperoxidase method. / Anderson, G. A.; Phillips, D. L.; Waldvogel, A. S.; Osburn, Bennie.
In: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, Vol. 1, No. 1, 01.01.1989, p. 45-49.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Detection of bluetongue virus in bovine fetuses using the avidin-biotin complex immunoperoxidase method.
AU - Anderson, G. A.
AU - Phillips, D. L.
AU - Waldvogel, A. S.
AU - Osburn, Bennie
PY - 1989/1/1
Y1 - 1989/1/1
N2 - The avidin-biotin complex immunoperoxidase technique was adapted for use in detecting bluetongue virus (BTV) antigens in BTV serotype 11-infected bovine fetuses. Fetuses were infected with BTV serotype 11 at 120 days of gestation and then removed 20 days later by Cesarean section. Blood and tissue samples were collected from each animal and used for virus isolation in embryonated chicken eggs, the immunofluorescent antibody test, and the avidin-biotin complex test. The avidin-biotin complex method successfully identified BTV antigens in both fresh and autolyzed fetal brains. Thus, the avidin-biotin complex immunoperoxidase method has potential as a possible procedure for diagnosing bluetongue disease in aborted bovine fetuses.
AB - The avidin-biotin complex immunoperoxidase technique was adapted for use in detecting bluetongue virus (BTV) antigens in BTV serotype 11-infected bovine fetuses. Fetuses were infected with BTV serotype 11 at 120 days of gestation and then removed 20 days later by Cesarean section. Blood and tissue samples were collected from each animal and used for virus isolation in embryonated chicken eggs, the immunofluorescent antibody test, and the avidin-biotin complex test. The avidin-biotin complex method successfully identified BTV antigens in both fresh and autolyzed fetal brains. Thus, the avidin-biotin complex immunoperoxidase method has potential as a possible procedure for diagnosing bluetongue disease in aborted bovine fetuses.
UR - http://www.scopus.com/inward/record.url?scp=0024481249&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0024481249&partnerID=8YFLogxK
U2 - 10.1177/104063878900100113
DO - 10.1177/104063878900100113
M3 - Article
C2 - 2562210
AN - SCOPUS:0024481249
VL - 1
SP - 45
EP - 49
JO - Journal of Veterinary Diagnostic Investigation
JF - Journal of Veterinary Diagnostic Investigation
SN - 1040-6387
IS - 1
ER -