Detection of bluetongue virus in bovine fetuses using the avidin-biotin complex immunoperoxidase method.

G. A. Anderson; D. L. Phillips; A. S. Waldvogel; Bennie Osburn

doi:10.1177/104063878900100113

Detection of bluetongue virus in bovine fetuses using the avidin-biotin complex immunoperoxidase method.

G. A. Anderson, D. L. Phillips, A. S. Waldvogel, Bennie Osburn

Research output: Contribution to journal › Article › peer-review

9 Scopus citations

Abstract

The avidin-biotin complex immunoperoxidase technique was adapted for use in detecting bluetongue virus (BTV) antigens in BTV serotype 11-infected bovine fetuses. Fetuses were infected with BTV serotype 11 at 120 days of gestation and then removed 20 days later by Cesarean section. Blood and tissue samples were collected from each animal and used for virus isolation in embryonated chicken eggs, the immunofluorescent antibody test, and the avidin-biotin complex test. The avidin-biotin complex method successfully identified BTV antigens in both fresh and autolyzed fetal brains. Thus, the avidin-biotin complex immunoperoxidase method has potential as a possible procedure for diagnosing bluetongue disease in aborted bovine fetuses.

Original language	English (US)
Pages (from-to)	45-49
Number of pages	5
Journal	Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc
Volume	1
Issue number	1
DOIs	https://doi.org/10.1177/104063878900100113
State	Published - Jan 1 1989
Externally published	Yes

ASJC Scopus subject areas

veterinary(all)

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Anderson, G. A., Phillips, D. L., Waldvogel, A. S., & Osburn, B. (1989). Detection of bluetongue virus in bovine fetuses using the avidin-biotin complex immunoperoxidase method. Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 1(1), 45-49. https://doi.org/10.1177/104063878900100113

Detection of bluetongue virus in bovine fetuses using the avidin-biotin complex immunoperoxidase method. / Anderson, G. A.; Phillips, D. L.; Waldvogel, A. S.; Osburn, Bennie.

In: Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, Vol. 1, No. 1, 01.01.1989, p. 45-49.

Research output: Contribution to journal › Article › peer-review

Anderson, GA, Phillips, DL, Waldvogel, AS & Osburn, B 1989, 'Detection of bluetongue virus in bovine fetuses using the avidin-biotin complex immunoperoxidase method.', Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, vol. 1, no. 1, pp. 45-49. https://doi.org/10.1177/104063878900100113

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Detection of bluetongue virus in bovine fetuses using the avidin-biotin complex immunoperoxidase method.

Abstract

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