Detection of bidirectional signaling during integrin activation and neutrophil adhesion

Stuart M. Altman, Neha Dixit, Scott I. Simon

Research output: Chapter in Book/Report/Conference proceedingChapter

2 Citations (Scopus)

Abstract

Neutrophil arrest and migration on inflamed endothelium is dependent upon a conformational shift in CD11a/CD18 (LFA-1) from a low to high affinity and clustered state which determines the strength and lifetime of bond formation with intracellular adhesion molecule 1 (ICAM-1). Cytoskeletal adaptor proteins kindlin-3 and talin-1 anchor clustered LFA-1 to the cytoskeleton and support the transition from neutrophil rolling to arrest. We employ microfluidic flow channels and total internal reflection fluorescence microscopy to evaluate the spatiotemporal regulation of LFA-1 affinity and bond formation that facilitate the transition from neutrophil rolling to arrest. Methodology is presented to correlate the relationship between integrin conformation, bond formation with ICAM-1, and cytoskeletal engagement and adhesion strengthening necessary to achieve a migratory phenotype.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages235-248
Number of pages14
Volume1124
ISBN (Print)9781627038447
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1124
ISSN (Print)10643745

Fingerprint

Lymphocyte Function-Associated Antigen-1
Neutrophil Activation
Integrins
Neutrophils
Plakins
Talin
Microfluidics
Cytoskeleton
Fluorescence Microscopy
Endothelium
Phenotype

Keywords

  • Adhesion strengthening
  • Cytoskeleton
  • G-protein coupled receptors
  • ICAM-1
  • Integrin
  • Intercellular adhesion molecule-1
  • Leukocyte function associated protein-1 (LFA-1)
  • Macrophage protein-1 (Mac-1)
  • Selectin

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Altman, S. M., Dixit, N., & Simon, S. I. (2014). Detection of bidirectional signaling during integrin activation and neutrophil adhesion. In Methods in Molecular Biology (Vol. 1124, pp. 235-248). (Methods in Molecular Biology; Vol. 1124). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-845-4_15

Detection of bidirectional signaling during integrin activation and neutrophil adhesion. / Altman, Stuart M.; Dixit, Neha; Simon, Scott I.

Methods in Molecular Biology. Vol. 1124 Humana Press Inc., 2014. p. 235-248 (Methods in Molecular Biology; Vol. 1124).

Research output: Chapter in Book/Report/Conference proceedingChapter

Altman, SM, Dixit, N & Simon, SI 2014, Detection of bidirectional signaling during integrin activation and neutrophil adhesion. in Methods in Molecular Biology. vol. 1124, Methods in Molecular Biology, vol. 1124, Humana Press Inc., pp. 235-248. https://doi.org/10.1007/978-1-62703-845-4_15
Altman SM, Dixit N, Simon SI. Detection of bidirectional signaling during integrin activation and neutrophil adhesion. In Methods in Molecular Biology. Vol. 1124. Humana Press Inc. 2014. p. 235-248. (Methods in Molecular Biology). https://doi.org/10.1007/978-1-62703-845-4_15
Altman, Stuart M. ; Dixit, Neha ; Simon, Scott I. / Detection of bidirectional signaling during integrin activation and neutrophil adhesion. Methods in Molecular Biology. Vol. 1124 Humana Press Inc., 2014. pp. 235-248 (Methods in Molecular Biology).
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