@inbook{6e1af2d5076044a6b33e4467fee3b607,
title = "Detection of bidirectional signaling during integrin activation and neutrophil adhesion",
abstract = "Neutrophil arrest and migration on inflamed endothelium is dependent upon a conformational shift in CD11a/CD18 (LFA-1) from a low to high affinity and clustered state which determines the strength and lifetime of bond formation with intracellular adhesion molecule 1 (ICAM-1). Cytoskeletal adaptor proteins kindlin-3 and talin-1 anchor clustered LFA-1 to the cytoskeleton and support the transition from neutrophil rolling to arrest. We employ microfluidic flow channels and total internal reflection fluorescence microscopy to evaluate the spatiotemporal regulation of LFA-1 affinity and bond formation that facilitate the transition from neutrophil rolling to arrest. Methodology is presented to correlate the relationship between integrin conformation, bond formation with ICAM-1, and cytoskeletal engagement and adhesion strengthening necessary to achieve a migratory phenotype.",
keywords = "Adhesion strengthening, Cytoskeleton, G-protein coupled receptors, ICAM-1, Integrin, Intercellular adhesion molecule-1, Leukocyte function associated protein-1 (LFA-1), Macrophage protein-1 (Mac-1), Selectin",
author = "Altman, {Stuart M.} and Neha Dixit and Simon, {Scott I.}",
year = "2014",
doi = "10.1007/978-1-62703-845-4_15",
language = "English (US)",
isbn = "9781627038447",
volume = "1124",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "235--248",
booktitle = "Methods in Molecular Biology",
}