Abstract
Neutrophil arrest and migration on inflamed endothelium is dependent upon a conformational shift in CD11a/CD18 (LFA-1) from a low to high affinity and clustered state which determines the strength and lifetime of bond formation with intracellular adhesion molecule 1 (ICAM-1). Cytoskeletal adaptor proteins kindlin-3 and talin-1 anchor clustered LFA-1 to the cytoskeleton and support the transition from neutrophil rolling to arrest. We employ microfluidic flow channels and total internal reflection fluorescence microscopy to evaluate the spatiotemporal regulation of LFA-1 affinity and bond formation that facilitate the transition from neutrophil rolling to arrest. Methodology is presented to correlate the relationship between integrin conformation, bond formation with ICAM-1, and cytoskeletal engagement and adhesion strengthening necessary to achieve a migratory phenotype.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 235-248 |
| Number of pages | 14 |
| Volume | 1124 |
| ISBN (Print) | 9781627038447 |
| DOIs | |
| State | Published - 2014 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1124 |
| ISSN (Print) | 10643745 |
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Keywords
- Adhesion strengthening
- Cytoskeleton
- G-protein coupled receptors
- ICAM-1
- Integrin
- Intercellular adhesion molecule-1
- Leukocyte function associated protein-1 (LFA-1)
- Macrophage protein-1 (Mac-1)
- Selectin
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Detection of bidirectional signaling during integrin activation and neutrophil adhesion. / Altman, Stuart M.; Dixit, Neha; Simon, Scott I.
Methods in Molecular Biology. Vol. 1124 Humana Press Inc., 2014. p. 235-248 (Methods in Molecular Biology; Vol. 1124).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Detection of bidirectional signaling during integrin activation and neutrophil adhesion
AU - Altman, Stuart M.
AU - Dixit, Neha
AU - Simon, Scott I.
PY - 2014
Y1 - 2014
N2 - Neutrophil arrest and migration on inflamed endothelium is dependent upon a conformational shift in CD11a/CD18 (LFA-1) from a low to high affinity and clustered state which determines the strength and lifetime of bond formation with intracellular adhesion molecule 1 (ICAM-1). Cytoskeletal adaptor proteins kindlin-3 and talin-1 anchor clustered LFA-1 to the cytoskeleton and support the transition from neutrophil rolling to arrest. We employ microfluidic flow channels and total internal reflection fluorescence microscopy to evaluate the spatiotemporal regulation of LFA-1 affinity and bond formation that facilitate the transition from neutrophil rolling to arrest. Methodology is presented to correlate the relationship between integrin conformation, bond formation with ICAM-1, and cytoskeletal engagement and adhesion strengthening necessary to achieve a migratory phenotype.
AB - Neutrophil arrest and migration on inflamed endothelium is dependent upon a conformational shift in CD11a/CD18 (LFA-1) from a low to high affinity and clustered state which determines the strength and lifetime of bond formation with intracellular adhesion molecule 1 (ICAM-1). Cytoskeletal adaptor proteins kindlin-3 and talin-1 anchor clustered LFA-1 to the cytoskeleton and support the transition from neutrophil rolling to arrest. We employ microfluidic flow channels and total internal reflection fluorescence microscopy to evaluate the spatiotemporal regulation of LFA-1 affinity and bond formation that facilitate the transition from neutrophil rolling to arrest. Methodology is presented to correlate the relationship between integrin conformation, bond formation with ICAM-1, and cytoskeletal engagement and adhesion strengthening necessary to achieve a migratory phenotype.
KW - Adhesion strengthening
KW - Cytoskeleton
KW - G-protein coupled receptors
KW - ICAM-1
KW - Integrin
KW - Intercellular adhesion molecule-1
KW - Leukocyte function associated protein-1 (LFA-1)
KW - Macrophage protein-1 (Mac-1)
KW - Selectin
UR - http://www.scopus.com/inward/record.url?scp=84898943615&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84898943615&partnerID=8YFLogxK
U2 - 10.1007/978-1-62703-845-4_15
DO - 10.1007/978-1-62703-845-4_15
M3 - Chapter
C2 - 24504956
AN - SCOPUS:84898943615
SN - 9781627038447
VL - 1124
T3 - Methods in Molecular Biology
SP - 235
EP - 248
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -